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IB Extended Essay in Biology

IB Extended Essay in Biology

Ratings: (0)|Views: 475|Likes:
Published by Momina Amjad
Research Question: To what extent is Miswak more
effective than toothpaste in reducing certain colonies
of oral bacteria, as observed by inoculation of pre and
post usage oral swabs?

Biolody was an HL subject for me.
Research Question: To what extent is Miswak more
effective than toothpaste in reducing certain colonies
of oral bacteria, as observed by inoculation of pre and
post usage oral swabs?

Biolody was an HL subject for me.

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Categories:Types, School Work
Published by: Momina Amjad on Aug 07, 2014
Copyright:Traditional Copyright: All rights reserved

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12/8/2013 BIOLOGY EXTENDED ESSAY
Comparative study on Miswak (Salvadora persica) and Colgate toothpaste effectiveness on the normal oral flora
 
Research Question: To what extent is Miswak more effective than toothpaste in reducing certain colonies of oral bacteria as observed by inoculation of pre and post usage oral swabs?
Momina Amjad
Candidate Number: 002223-0028 Word Counts Abstract: 288 Essay:
 
3,973
 
 Abstract
Salvadora persica
or miswak has been used for a long time in Middle East and North Africa regions by Muslims for maintenance of oral hygiene. I wanted to investigate whether miswak actually had an antimicrobial effect and if this was greater than or less than that of toothpaste. Subsequently, I also wanted to determine which specific microorganisms were reduced the most and the implications of miswak on tooth decay prevention and worldwide healthcare costs associated with that. Therefore this research question was formulated for the Extended Essay:
To what extent is Miswak more effective than toothpaste in reducing certain colonies of oral bacteria, as observed by inoculation of pre and post usage oral swabs?
A sample of 10 people was selected for this study, 5 for the miswak trials and 5 for toothpaste trials. An oral swab was taken by the subjects and then they were asked to brush their teeth with either miswak or toothpaste for two minutes, after which another swab was taken. These swabs were later inoculated in Blood agar, Chocolate agar, MacConkey agar and Sabouraud agar to qualitatively observe (using an ACFOR scale) the reduction of specific colonies between the pre and post treatment samples. Gram staining was then performed to help identify various bacterial species. The results of the experiments indicated that miswak was more effective on cariogenic (tooth decay causing) species of bacteria such as
Streptococcus mutans
. Toothpaste, on the other hand, was more effective on potential pathogens like
Staphylococcus aureus
. Miswak and toothpaste were equally effective on gamma hemolytic colonies of
Neisseria
 species. More research has to be done with quantitative aspects in order to be conclusive about these findings and integrate miswak as a major means of tooth decay prevention.
 
 Acknowledgements
I would like to thank the Ministry of Health- North Batinah region, Oman, for letting me carry out my experiment and use all the required resources at the Pathology department in Sohar Hospital. I would also like to thank Naima Salem Khalfan Al Raisi and Ahmed Abdullah Mohammed Omer for all of their support and time, during and after their working hours, and for teaching me all the practical skills in microbiology that were required in this research.

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Momina Amjad added this note
Please note: 1) This is intended to be used as a guide to help you start. If you take out chunks of text or ideas directly it would count as plagiarism and would cost you your diploma. 2) This extended essay is in line with the requirements of the syllabus upto 2014 examinations. The criteria may change after that, consult your EE guide. Feel free to comment if you have queries.
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