UNIVERSITI PUTRA MALAYSIA

FAKULTI PERUBATAN DAN SAINS KESIHATAN

BIOCHEMISTRY UNIT
DEPARTMENT OF BIOMEDICAL SCIENCES
FACULTY OF MEDICINE AND HEALTH SCIENCES
SBP3201
BASIC MEDICAL BIOCHEMISTRY

Title: Laboratory Buffers
Date: 13 July 2009

Lecture: Dr. Abdah Md Akim

Group Member:
1. Lee Siew Siew 152157
2. Rahmatulhidayani Binti Muhammad Thahir 152252
3. Noreen Nadia Bte Bagia 154073
4. Noor Athia Adhwa Bte Norazlan 153833
5. Noratirah Shazlin Binti Muhamad Adyab 153843



PROBLEMS
1. Calculate the pH in the titration of25 ml of 0.10 M acetic acid by sodium hydroxide
after the addition to the acid solution of

a) 10 ml of 0.10 M NaOH

[ ]
[]

]
[

CH
3
COOH NaOH CH
3
COONa
Initial mole of
(mole)

(excess)

0
Mole after
reaction
2.5 X 10
-3
1 X 10
-3

= 1.5 X 10
-3

0 1 X 10
-3
b) 25 ml of 0.10 M NaOH







Salt CH
3
COONa undergoes hydrolysis:

8.72

28 . 5 14
28 . 5
10 27 . 5 log
10 27 . 5 ] [
10 78 . 2 ] [
05 . 0
] [
10 56 . 5
] [
] ][ [
10 56 . 5
10 8 . 1
10 1
6
6
11 2
2
10
3
3
10
5
14
pH
pOH
OH
OH
OH
COOH CH
OH COOH CH
K
K
K
K
b
a
W
b


CH
3
COOH NaOH CH
3
COONa
Initial mole of
(mole)

0
Mole after
reaction
0 0

c) 35 ml of 0.10 M NaOH

CH
3
COOH NaOH CH
3
COONa
Initial mole of
(mole)

0
Mole after
reaction(mole)
0

8.07

93 . 5 14
93 . 5
10 18 . 1 log
10 18 . 1 ] [
10 39 . 1 ] [
10 5 . 2
] [
10 56 . 5
] [ ]
] [
] ][ [
10 56 . 5
10 8 . 1
10 1
6
6
12 2
3
2
10
3
3
10
5
14
pH
pOH
OH
OH
OH
OH COOH [CH
COO CH
OH COOH CH
K
K
K
K
3
b
a
W
b
2. Which of the following solutions are buffer systems?
a. KH
2
PO
4
/H
3
PO
4

This is an acidic buffer solution which the weak acid is H
3
PO
4
and the KH
2
PO
4
is
salt of weak acid of H
3
PO
4
. H
3
PO
4
is weak acid which dissociate partially form
H+ and H
2
P

KH
2
PO
4
is salt that dissociate fully in the solution.

When a little amount of acid is added into the system, H
+
ion from the acid will
react with

from dissociation of salt to form H

3
PO
4
. so there is not
change in the pH of the system.

When a little amount of base is added into the system the OH
-
from the base will
react with H
+
from the dissociation of acid. Water is formed. So there is not
change in the pH of the system.

Adding of both acid or base in this system causes no much change in the pH of
the system. Therefore KH
2
PO
4
/H
3
PO
4
is a buffer system
.

b. NaClO
4
/HClO
4

This is also an acidic buffer solution which the weak acid is HClO
4
and the
NaClO
4
is salt weak acid of HClO
4
HClO
4
is weak acid which dissociate
partially form H
+
and

NaClO
4
is salt that dissociate fully in the solution

When a little amount of acid is added into the system, H
+
ion from the acid will
react with

from dissociation of salt to form HClO

4
. So there is no change
in the pH of the system.

When a little amount of base is added into the system the OH
-
from the base will
react with H
+
from dissociation of acid. Water is form. So there is not change in
the pH of the system.

Adding of both acid or base in this system causes no much change in the pH of
the system. Therefore NaClO
4
/HClO
4
is a buffer system.

c. C
5
H
5
N/C
5
H
5
NHC
This is not a buffer solution. (C
5
H
5
N) pyridine is the strong base, when it react
with acid (HCl) it will form perpetual salt,

Therefore this is not a buffer solution.


3.
a) Calculate the pH of a buffer system containing 1.0 M CHCOOH and 1.0 M
CHCOONa
4.74

1
1
log 10 8 . 1 log
[
log
5
[acid]
base] conjugate
pK pH
a


b) What is the pH of the buffer system after the addition of 0.10 mole of gaseous
HCl to 1L of the solution? Assume that the volume of the solution does not
change when the HCl is added.

]
[

[ ]
[]

4. A buffer solution is prepared by mixing 400 ml of 1.50 M HCl solution with 600 ml
of 0.10 M NH solution.
a) Calculate the pH of the buffer
[

8.26

74 . 5 14
14
74 . 5
06 . 0
6 . 0
log 10 8 . 1 log
] [
[
log
5
pOH pH
base
acid] conjugate
pK pOH
b


b) Calculate the new pH of the buffer after the addition of 2.0 ml of a 0.1 M HCl

NH
3
NH
4
Cl
Initial mole (mole)

Mole after adding
HCl(mole)

Concentration after
adding HCl (M)
0.0598 0.6004

8.25

75 . 5 14
14
75 . 5
0598 . 0
6002 . 0
log 10 8 . 1 log
] [
[
log
5
pOH pH
base
acid] conjugate
pK pOH
b


5.
?
1
5026 . 0
] [
[
5026 . 0
] [
[
29874 . 0
10 1 . 7 log 85 . 2
] [
log
] [
[
log 10 1 . 7 log 85 . 2
] [
[
log
4
4

acid
base] conjugate
acid
base] conjugate
acid
base] [conjugate
acid
base] conjugate
acid
base] conjugate
pK pH
a

To prepare NaF/HF buffer with a pH of 2.85 NaF/HF at ratio 0.5026/1 must be used.

6. How many ml of a0.610 M NaOH solution are needed to neutralize 20.0 ml of a
0.245 M HSO solution?

7. Draw the structures of the following amino acids in acidic, basic and neutral
solutions.
a) Glycine
b) Alanine



Amino acid Glycine Alanine
Neutral

Acidic

Basic