Welcome to Scribd, the world's digital library. Read, publish, and share books and documents. See more
Download
Standard view
Full view
of .
Look up keyword or section
Like this
0Activity
P. 1
Micro Man

Micro Man

Ratings: (0)|Views: 0 |Likes:
Published by vk100
yyry
yyry

More info:

Categories:Types, School Work
Published by: vk100 on Aug 19, 2014
Copyright:Traditional Copyright: All rights reserved

Availability:

Read on Scribd mobile: iPhone, iPad and Android.
download as PDF, TXT or read online from Scribd
See more
See less

08/19/2014

pdf

text

original

 
Cover Art: Immunofluorescence image of several
Cryptosporidium parvum 
 oocysts provided by H.D.A Lindquist, USEPA
 
Laboratory Manual for the Microbiological AnalysesoPublic Drinking Water 2001
Donald E. LaSotaTodd W. BidlacJames W. Evans
Christopher JonesOhio EPA Director Bob TafGovernor of Ohio
 
ii
T A B L E O F C O N T E N T ST A B L E O F C O N T E N T S
Chapter I ............................................................ 1Certification Overview ............................................ 1Requirements for Certification....................................... 2Laboratory Approval Status ......................................... 2Proficiency Testing ............................................... 2Drinking Water Analysis for Other Entities ............................. 5 The Microbiological Water Testing Course ............................. 6Chapter II ........................................................... 7Sampling....................................................... 7 Transportation and Storage .................................... 7Record of Laboratory Examination .............................. 7Instructions for submitting the Microbiological SSR ....................... 7Collection of Samples ............................................ 10Chapter III .......................................................... 13Laboratory Facilities ............................................. 13Laboratory Space .......................................... 13Laboratory Construction and Remodeling ....................... 13Laboratory Construction ..................................... 14EquipmentAbsorbent Pads ........................................... 14Autoclave ............................................... 14Balance ................................................. 15Culture Tubes............................................. 15Forceps ................................................. 15Hot Air Sterilizing Oven ..................................... 16Incubator ................................................ 16Inoculating Equipment ...................................... 16Media Preparation Utensils................................... 17Membrane Filters .......................................... 17Membrane Filtration Units ................................... 17Microscope and Lamp ...................................... 17
 
iiiPetri Dish Containers ....................................... 18Petri Dishes .............................................. 18pH Meter ................................................ 18Pipet Containers........................................... 19Pipets ................................................... 19Refrigerator .............................................. 19Sample Containers ......................................... 19 Thermometers ............................................ 20Calibration Procedures ................................ 21MMO-MUG Test Vessels .................................... 22Resistivity/Conductivity Meter ................................ 22 Timers .................................................. 22Fecal Coliform Incubator .................................... 22UV Light ................................................ 23Chapter IV .......................................................... 24Materials and Media Preparation .................................... 24Cleaning Glassware ........................................ 24Sterilization of Materials..................................... 24Microbiologically Suitable Water .............................. 25Buffered Rinse Water ....................................... 26pH Measurements ......................................... 27Sterilization of Media ....................................... 27Storage of Media, Reagents, and pH Buffers ..................... 27Media Quality Control ...................................... 28Culture Methods .......................................... 29Chapter V .......................................................... 31Culture Media Specifications and Preparation .......................... 31Lauryl Tryptose Broth ....................................... 31Brilliant Green Lactose Bile Broth.............................. 32m-Endo Medium .......................................... 33EC Broth................................................. 35BHI Broth................................................ 36 TSB .................................................... 37Chapter VI .......................................................... 38Membrane Filter (MF) Procedures ................................... 38Filtering Procedures ....................................... 38Membrane Filter (MF) Quality Control ............................... 39Incubation .................................................... 39Filtration Volumes............................................... 39Potable water (finished drinking water). .............................. 39

You're Reading a Free Preview

Download
/*********** DO NOT ALTER ANYTHING BELOW THIS LINE ! ************/ var s_code=s.t();if(s_code)document.write(s_code)//-->