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1. As a part of this research project, the student directly handled, manipulated, or interacted with (check ALL) that apply.
Human Subjects pathogenic agents recombinant DNA

Non human vertebrate animal controlled substances human/animal tissue

2. Student independently performed all procedures as outlined in this abstract Yes No
3. This project was conducted at a Regulated Research Institutions. Yes No
4. Is this a project continuation? Yes No
I /We hereby certify that the above statements are correct and the information provided in the Abstract is the result of
one years research. I/We also attest that the above properly reflects my/our work.

The Extraction of Bioethanol from Pineapple (Ananas comosus) Peelings
Through Simultaneous Saccharification and Fermentation Using the Yeast
Saccharomyces cerevisiae

Diomel Escutin, Renzo Laporno, Karl Sardina, Andrei Ong, Yawen Claudio, Elijah
Dayon

PAREF Westbridge School Inc., (Magsaysay Village, La Paz, Iloilo City)


On average, 435,000 metric tons of pineapples are produced annually
in the Philippines, which is one of the countrys leading commercial fruit
products. However, there are a lot of unused excess parts of the pineapple,
notably the peelings, which are considered as waste and contribute to the
countrys garbage problem.

There is a global oil crisis, as the demand for petroleum increases each
year while our supplies are rapidly being depleted. Bioethanol, a principle
biofuel, is a natural alternative to gasoline.

One solution to both dilemmas is to produce bioethanol from
pineapple plant peelings. This will be made possible by extracting the glucose
content of the peelings and fermenting it through the process of Simultaneous
Saccharification and Fermentation (SSF) using the yeast sample. Peel samples,
coming from Ananas comosus, will undergo a series of physical and chemical
analyses to determine the glucose content, which will be utilized to yield
bioethanol. The SSF process will be manipulated in terms of fermentation time
and buffer level used to determine the best variables for biofuel production.