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Biol 101 Sample QuestionsTopic 6: Biotechnology
I. Multiple-Choice Questions
1. Biotechnology is presently being used to do which of the following?A) produce vaccinesB) correct defects in human germ cellsC) produce human gene productsD) only A and C correctE) A, B, and C are correct2. What is the function of restriction enzymes?A) add new nucleotides to the growing strand of DNAB) join nucleotides during replicationC) cut nucleic acids at specific sitesD) join nucleotides during transcriptionE) repairs breaks in sugar-phosphate backbones3. Identify the true statement(s):A) DNA directs the synthesis of proteins, which in turn directs the synthesis of RNA.B) Ribosomes, which are the site of DNA replication, are formed from two subunits, each composed of rRNA and protein.C) The ribosome’s role in protein synthesis is to bring the tRNA molecule and mRNA moleculetogether.D) B and CE) none of the above4. If you discovered a bacterial cell that had no restriction enzyme, which of the following would youexpect to happen?A) the cell would be unable to replicate its DNAB) the cell would create incomplete plasmidsC) the cell would be easily infected and lysed by virusesD) the cell would become a parasiteE) both A and D would occur5. The anticodon for GCG is:A) AUAB) UAUC) CCTD) CGCE) CGU
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II. Identify the following statements as TRUE or FALSE.
a) The polymerase chain reaction is important because it allows us to makemany copies of a targeted segment of DNA.
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b) A bacterial cell that contained no restriction enzymes would be unable toreplicate its DNA.
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c) Human Genome Project was very successful in determining the functions of over 80% of the genes located in 46 chromosomes.
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d) A molecule that contains the DNA from two different organisms is called arecombinant DNA.
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e) DNA ligase is the enzyme that cuts DNA, creating the sticky ends of restriction fragments.
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f) DNA molecule is a double helix where the strands are held together by themonosaccharide deoxyribose.
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g) During the process of transcription, in making RNA the nitrogenous baseuracil in the RNA pairs with the nitrogenous base thymine of the DNA.
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h) Stem cells are found in bone marrow.
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i) Translation can be defined as the making of protein using mRNA and tRNA.
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j) The specific bonding pattern between the nitrogenous bases of two DNAstrands is called complementary base pairing.
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k) Adenine and guanine are two-ring structures called purines and thymine andcytosine are one-ring structures called pyrimidines.
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l) A tRNA molecule is made up of two strands of repeating nucleotide unitswhile mRNA's are single stranded.
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m) If one segment of a DNA molecule has a code of ATACGGATC itscomplementary strand should have a code of TAAGCCTAG.
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n) Stem cells are one of the common sources of therapeutic cloning.
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o) An organism containing a gene from another species is called a clone.
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Biol 101 Sample Questions Topic 6: Biotechnology
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