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Lizet Enriquez B3 Biology Ap

Enzymes in ActionAbstract

The labs purpose is to teach you how to measure the effects of changes in temperature, enzymeconcentration and substrate concentration and how these affect the reactions of enzymes in acontrolled experiment. Enzymes are in our bodies and this is being shown in the experimentwith the liver reacting in the hydrogen peroxide. The methods in this experiment must befollowed as directed. The results show what the hypothesis had stated. It is supporting thehypothesis by showing that the factors used in the lab are affecting the enzyme-substratereaction. This lab is teaching you that enzymes are in living things even in humans. It candenature by temperature, pH, and enzyme and substrate concentration. Time also has a greateffect on a reaction in an enzymatic solution. This is shown in the experiment.

Introduction

Enzymes are proteins that are produced by living cell that act as biochemical catalysts. Aseverybody knows an enzyme must bind to a substrate at the active site in order to make a productof the reaction formed. There are several ways an enzyme action can be affected. Some of thefactors are salt concentration, pH, temperature, and if there are any activations or inhibitorsacting on the enzyme. Many of these factors can make an enzyme denature or slow down makingthe reaction inactive. The purpose of this lab is to seeing how enzyme catalysis works. If you put

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Lizet Enriquez B3 Biology Ap the catalase solution in different things such as hydrogen peroxide mixed with water then you aregoing to see different reactions occurring.

Materials and Methods

For the first test you will need: Hydrogen peroxide, 3 medicine cups, catalase solution, 1 testtube, test tube rack, cube of liver, 12mL syringe, 1 mL pipette, pencil and paper. For the secondtest you will need: Hydrogen peroxide, 8 medicine cups, 6mL syringe, 1 mL pipette, 1 mL of distilled water in medicine cups, Sulfuric acid, 12mL syringe, and Potassium permanganate.Goggles and gloves must be used in the lab at all times.Test of Catalase Activity 11.Transfer 10mL of Hydrogen Peroxide into a medicine cup2.Then add 1 mL of catalase solution

After observing the reaction and recording the results clean all materials and drythemTest of Catalase Activity 21.Put 5 mL of catalase solution into a test tube2.After that place the test tube in the water bath for 5 minutes

Put 10 mL hydrogen peroxide into a medicine cup4.Take 1 mL of the cooled boiled catalase solution and add it to the medicine cup5.After recording the results clean all materials and dry themTest of Catalase Activity 31.Cut 1 cm of a liver and macerate it

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Lizet Enriquez B3 Biology Ap 2.Put it in a medicine cup containing 10 mL of hydrogen peroxide3.After recording the results clean all materials and dry themBase Line Test 11.Put 10 mL of hydrogen peroxide in a medicine cup2.Add 1 mL of distilled water 3.Then add 10 mL of sulfuric acid4.Mix the solution well5.Remove a 5 mL sample6.Put the sample into a medicine cup7.Place the medicine cup under a piece of white paper

Using the syringe add 5 mL of potassium permanganate one drop at a time after eachdrop gently swirl and keep adding drops until you get a pink or brown color in thesolution.9.Record the results

ResultsTest of catalase activity 1 ResultsEnzyme in reactionSubstrate in reactionProduct in reactionObservation

Catalase solutionHydrogen Peroxide OxygenThe presence of bubblesFor the test of catalase activity 2 the solution didn’t bubble as much as activity 1 because the boiling denatures the catalase. The test of catalase activity 2 it is bubbling more than activity 1 or