1

Postlab Notes in Biochem

Act. 5: Color test for proteins and specific Amino acids
Classification and Structure of amino acids
-Nonessential amino acids are those that are synthesized by mammals, while the essential amino
acids must be obtained from dietary sources.
Classification - Amino acid classification according to reactive group or R group
1. Aliphatic - Glycine, Alanine, Valine, Leucine, Isoleucine
2. Aromatic - Phenylalanine, Tyrosine, Tryptophan
3. OH- - Serine, Threonine
4. Acidic - Aspartic Acid, Glutamic Acid
5. Acid amide - Aspargine, Glutamine
6. Basic - Arginine, Lysine, Histidine
7. Sulphur - Cystine, Methionine
8. Cyclic - Proline
Classification - Dependent in the property of R group
1. Non polar or Hydrophobic - Glycine, Alanine, Valine, Leucine, Isoleucine, Proline
2. Polar uncharged - Phenylalanine, Tryptophane, Tyrosine
3. Polar charged - Serine, Threonine (OH-), Cystine, Methionine (sulphur), Aspargine, Glutamine
(acid amide)
4. Positively charged - Aspartic Acid, Glutamic Acid
5. Negatively charged - Arginine, Lysine, Histidine




















2

Experiment 7- Denaturation of Proteins





3









4

Act. 10 Test for Carbohydrates
Fischer projections
Not all of the following monosaccharides are found in naturesome have been
synthesized:
Aldoses
Aldotrios
e

D-Glyceraldehyde
Aldotetro
ses

D-Erythrose

D-Threose
Aldopento
ses

D-Ribose

D-Arabinose

D-Xylose

D-Lyxose
Aldohexos
es

D-
Allose

D-
Altrose

D-
Glucose

D-
Mannose

D-Gulose

D-Idose

D-
Galactos
e

D-Talose

5
Ketoses
Ketotriose

Dihydroxyacetone
ketotetrose

D-Erythrulose
ketopentoses

D-Ribulose

D-Xylulose
ketohexoses

D-Psicose

D-Fructose

D-Sorbose

D-Tagatose