University of Colorado Denver

Guidelines for Fluid Administration and Blood Collection

Modified: 1/10/2011
Maximum Injectable Volumes Per Route of Injection
Species IP IM SC IV ID
Mouse 2.0 mL 0.05 mL 3 mL 0.2 mL N/A
Rat 5 mL 0.1 mL 5 mL 0.5 mL 0.05 mL
Guinea Pig 10 mL 0.3 mL 5 mL 0.5 mL 0.1 mL
Hamster 4 mL 0.1 mL 3 mL 0.3 mL 0.05 mL
Gerbil 3 mL 0.1 mL 3 mL 0.3 mL 0.05 mL
Rabbit 50 mL 1.5 mL 100 mL 5.0 mL 0.1 mL
(Abbreiations! "P#intra$eritoneal% "M#intramus&ular' ()#sub&utaneous%
"*#intra+ermal% ",#intraenous-
Common Venous Access Points for Injection or Blood Collection
Mouse: subman+ibular ein' +orsal $enile ein' lateral sa$.enous ein'
lateral tail ein
Rat: sublingual ein' /ugular ein' +orsal $enile ein' lateral sa$.enous ein'
lateral tail ein
Guinea pi: sublingual ein' +orsal $enile ein' lateral metatarsal ein
!amster: /ugular ein' lateral sa$.enous ein
Gerbil: /ugular ein' lateral sa$.enous ein' lateral tail ein
Rabbit: lateral ear ein' /ugular ein' &e$.ali& ein' lateral sa$.enous ein
Blood Collection Volumes
0.e total bloo+ olume in an a+ult mammalian laborator1 animal s$e&ies is
a$$ro2imatel1 34 o5 bo+1 6eig.t. 0.e olume o5 bloo+ t.at &an be remoe+
at one &olle&tion s.oul+ not e2&ee+ 104 o5 total bloo+ olume 6it.out 5urt.er
&are. 782am$le! 25 g mouse # 0.135 mL' 200 g rat # 1.4 mL' 35 g .amster #
0.52 mL' 4 9g rabbit # 2: mL;.
154 o5 bloo+ olume &an be remoe+ at one time i5 &olle&te+ slo6l1'
an+ 6arm re$la&ement <ui+s are a+ministere+ ()' "P' or ", to re$la&e
t.e bloo+ olume &olle&te+.
=or re$eate+ bloo+ sam$ling' u$ to 3.54 o5 t.e total bloo+ olume &an
be ta9en $er 6ee9' or 104 o5 t.e total bloo+ olume ma1 be remoe+
eer1 t6o 6ee9s.
0o &olle&t bloo+ at a more 5re>uent interal or in larger olumes' a
.ig.er leel o5 $.1siologi& monitoring 6ill be re>uire+' an+ a$$roe+
b1 t.e "nstitutional Animal ?are an+ @se ?ommittee.
More $re&ise +ata e2ists on t.e total olume o5 bloo+ t.at e2ists in t.e t1$i&al
A*@L0 laborator1 animal s$e&ies. =or t.e &olle&tion o5 bloo+' 104 o5 total
bo+1 bloo+ olume &an also be &al&ulate+ base+ on t.e 5ollo6ing alues%
Mouse! 1A2.35 mL% Rats! B4 mL/9g% Guinea Pig! 35 mL/9g% Hamster! 3: mL/9g%
Gerbil! BBA3: mL/9g% Rabbit! 53AB5 mL/9g
"ral#Gastric Ga$ae
Cral gaage is t.e a+ministration o5 <ui+s +ire&tl1 into t.e lo6er eso$.agus
or stoma&. using a 5ee+ing nee+le intro+u&e+ into t.e mout. an+ t.rea+e+
+o6n t.e eso$.agus. =ee+ing nee+les .ae a stainless steel ballAti$ t.at
ma9es t.em atraumati& on +eli&ate oral an+ eso$.ageal tissues an+ re+u&es
t.e &.an&e o5 intro+u&ing t.e ballAti$ in t.e tra&.ea. 0.e gui+eline 5or olume
o5 a+ministration is 10 mL/9g in t.e mouse an+ 20 mL/9g bo+1 6eig.t in t.e
rat.
References
1. Hra$9ie6i&D' E.' Me+ina' L.' an+ Holmes' *. ?lini&al Laborator1 Animal
Me+i&ine! An "ntro+u&tion. 2
n+
8+. "o6a (tate Press' 1FF:.
2. Ha69' ?.0.' Lear1' (.' Morris' 0. =ormular1 5or Laborator1 Animals. 2
n+
8+.
"o6a (tate @niersit1 Press' 1FFF.
3. Gol5enso.n' (. an+ Llo1+' M. Han+boo9 o5 Laborator1 Animal Management
an+ Gel5are. 2
n+
8+. Hla&96ell (&ien&e' 1FF:
4. Hulin' M. et al. Laborator1 Animal *ata! )ui&9 Re5eren&e Gui+e =or
Resear&.ers. Ameri&an Asso&iation o5 Laborator1 Animal (&ien&e' 2002.
5. 666.&iti$rogram.org% +o6nloa+e+ F/22/2010
B. 666.aalaslearninglibrar1.org% +o6nloa+e+ 10/4/2010