Plant Growth Regulator g/L g/100 mL
Benzylaminopurine(BAP)1.000 1 (100/1000) = 0.1 gNaphthaleneacetic acid(NAA)1.000 1 (100/1000) = 0.1 g
Macronutrients are normally required in millimolar (mM) quantities in most plantmedia. The macronutrient normally contains high amount of nitrogen fixing agents inthe form of ammonium (NH
) and nitrate ions (NO
) ions. Apart from nitrogenicsubstances, potassium, magnesium, calcium, sulphur and phosphorus are also addedas macronutrient in the form of diluted salts. Also, macronutrients serve ascomponents for structural and protoplasmic tissue.
Micronutrients are needed in a very small amount. The low requirement of micronutrients can be accounted for participation of these elements in enzymaticreactions and as constituents of growth hormones. The micronutrient is the mixtureof boron, cobalt, manganese, molybdenum, copper and zinc. Excessive amountadded to the medium can cause suffocation and premature death of the explantsused.
Ferum is required in metabolic functions such as nitrogen fixation, photosynthesisand electron carri
er during respiration’s electron transfer process. It is usually
present in the form of FeSO
O and Na
EDTA. In some cases, ferum is preparedtogether with micronutrient. Ferum oxidises in the presence of sunlight.Furthermore, high concentrations will cause precipitation to occur. Therefore,preparing ferum and storing the stock solution in dark environment can prolong theshelf life of ferum stock solution.
Vitamin is essential as it is involved in carbohydrate metabolism and the biosynthesisof some amino acids. Normally thiamine is deemed as the most important vitaminand it is introduced as thiamine hydrochloride. Other vitamins like pyridoxine,nicotinic acid are added as well. Certain plant species requires special requirementsof vitamin like biotin, riboflavin, folic acid and more. Vitamins are associated withmetabolic activity of the plant. Therefore, to have good yield, sufficient amount of vitamin should be added to the MS medium.
Plant growth regulators
Plant growth regulator (PGR), functions in initiating the root and shoot developmentof explants and embryos. They also stimulate cell division and expansion. Certainparts of plants have plant growth regulators readily available in the explants. In casesof PGR absence, PGR supplemented through medium enables growth of the