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Genes in a Bottle Instruction Manual

Genes in a Bottle Instruction Manual

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Published by dnajen

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Published by: dnajen on Dec 30, 2009
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06/16/2013

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Biotechnology Explorer
Genes in a Bottle KitDNA Extraction Module
Catalog Number166-2000EDUDNA necklace module (166-2200EDU)must be purchased separately.explorer.bio-rad.com
See individual components for storage temperature.
For Technical Service Call Your Local Bio-Rad Office or in the U.S. Call
1-800-4BIORAD
(1-800-424-6723)
Duplication of any part of this document is permitted for classroom use only.
 
Capture Your Essence!
Whether it’s being cloned, sequenced, fingerprinted, mapped, or genetically engineered,DNA has become an everyday topic in the media and the classroom. Introduce your students to the molecular framework of biology —with their own essence! Your studentscan capture, preserve, and even bottle their own DNA.How do scientists separate pure DNA from cells composed mainly of lipids, proteins,carbohydrates, and salts? Membranes are first ruptured with detergents to release DNAinto a solution; then proteins and other organic molecules are digested and separated whilethe DNA remains intact. The DNA is finally collected by precipitation in a form that can bemanipulated as desired.With this simple laboratory activity, students gain practical knowledge by conducting areal-world laboratory procedure that is used to extract DNA from many different organismsfor a variety of applications. Your students will extract genomic DNA from their own cheekcells and watch it precipitate from solution as floating white strands. Using the DNAextraction module (166-2200EDU), the DNA strands are then easily collected and transferredto a glass vial, and the vial is fashioned into a necklace.This kit is suitable for students from 5th grade through college and minimal backgroundknowledge is required. This laboratory activity can be performed at any point during atypical biology or life science course, when topics such as cells, cell structure, mitosis andmeiosis, genetics, and DNA technology are discussed.For students learning about the molecular framework of biology for the first time, DNA isabstract and intangible. This procedure makes the invisible visible —seeing their own DNAmakes it real. Illustrations of DNA and additional laboratory activities develop students’understanding of DNA’s function as the genetic blueprint, and help students comprehendthis previously invisible substance of life.This kit provides learning opportunities for all levels of instruction. The activity is designedfor any classroom environment and requires no specialized equipment or stains. For secondary and college level instruction, lessons on DNA structure and function, cellstructure, and enzyme function can be introduced or reinforced with this laboratory activity.For middle school students, it’s a perfect introduction to the exciting world of DNA science.We welcome your comments and suggestions. Have fun!Nebbie Idris, PhDBiotechnology Explorer Product Manager 
 
Table of Contents
Teacher’s Guide
Kit Inventoryand Supply Checklist
....................................................................1
Overview for the Teacher
....................................................................................2Why Teach DNA Extraction?............................................................................2Intended Audience............................................................................................2Curriculum Fit....................................................................................................3Recommended Student Background................................................................3Activity Timeline................................................................................................3Safety Issues....................................................................................................3Keys to Success................................................................................................3Volume Measurements....................................................................................3Background and Fundamentals for StudentsBasic Level Introduction............................................................................4Advanced Level Introduction....................................................................6
Teacher’s Laboratory Guide
Suggested Lesson Flow and Implementation Timeline..........................................8Teacher’s Advance Preparation............................................................................8Workstation Checklist....................................................................................10Quick Guide (Graphic Laboratory Protocol)........................................................11
Student Manuals
Basic Level Student Manual
..............................................................................14Introduction and Focus Questions..................................................................15Workstation Checklist....................................................................................18Instructions for DNA Extraction and Precipitation..........................................18
Advanced Level Student Manual
......................................................................23Introduction and Focus Questions..................................................................24Workstation Checklist....................................................................................29Instructions for DNA Extraction and Precipitation..........................................29
Extension Activities
Dry Laboratory Demonstration of DNA Extraction..........................................34Microscropic Observation and Nuclear Staining of Cheek Cells....................34
Answers to Focus Questions
Basic Instruction..............................................................................................35Advanced Instruction....................................................................................36

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