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Sub Cell 96 Instructions

Sub Cell 96 Instructions

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Published by: dnajen on Dec 30, 2009
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06/22/2010

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Sub-Cell
®
Model 96 andModel 192Agarose GelElectrophoresis SystemsInstruction Manual
Catalog Numbers170-4500 through 170-4511
For Technical Service Call Your Local Bio-Rad Office or in the U.S. Call
1-800-4BIORAD
(1-800-424-6723)
 
Warranty
Bio-Rad Laboratories warrants the Sub-Cell Model 96 and Model 192 electrophoresis systems againstdefects in materials and workmanship for 1 year. If any defects occur in the instrument during thiswarranty period, Bio-Rad Laboratories will repair or replace the defective parts free. The followingdefects, however, are specifically excluded:1.Defects caused by improper operation.2.Repair or modification done by anyone other than Bio-Rad Laboratories or an authorizedagent.3.Use of fittings or other spare parts supplied by anyone other than Bio-Rad Laboratories.4.Damage caused by accident or misuse.5.Damage caused by disaster.6.Corrosion due to use of improper solvent or sample.This warranty does not apply to parts listed below:1.Platinum Electrode WiresFor any inquiry or request for repair service, contact Bio-Rad Laboratories after confirming themodel and serial number of your instrument.To insure the best performance from the Sub-Cell electrophoresis systems, become fully acquaintedwith these operating instructions before use. Bio-Rad recommends that you first read these instructionscarefully. Assemble and disassemble the unit completely without casting a gel. After these prelimi-nary steps, you should be ready to cast and run a gel.Bio-Rad also recommends that all Sub-Cell system components and accessories be inspected fordamage, cleaned as recommended in this manual and rinsed thoroughly with distilled water beforeuse. Record the following for your records:
ModelCatalog No.Date of DeliveryWarranty PeriodSerial No.Invoice No.Purchase Order No.
 
Table of Contents
Page
Warranty Information ..........................................................................
Inside Front Cover
Section 1 General Information....................................................................................1
1.1 Introduction................................................................................................................11.2 Safety..........................................................................................................................11.3 List of System Parts....................................................................................................31.4 Specifications.............................................................................................................5
Section 2 Operating Instructions................................................................................6
2.1 DNA Gel Preparation.................................................................................................62.2 Comb Set-up...............................................................................................................72.3 Casting Agarose Gels.................................................................................................82.4 Recirculation Ports...................................................................................................112.5 Electrophoresis.........................................................................................................122.6 Nucleic Acid Staining and Visualization.................................................................132.7 Note on Blotting.......................................................................................................14
Section 3 Gel and Electrophoresis Reagents Preparation......................................14
3.1 Electrophoresis Buffer Preparation..........................................................................143.2 DNA and RNA Gel Preparation..............................................................................153.3 RNA Sample Preparation.........................................................................................153.4 DNA and RNA Sample Loading Dye.....................................................................163.5 Gel Staining Solution...............................................................................................16
Section 4 Care and Maintenance..............................................................................16
4.1 Cleaning Sub-Cell System Components..................................................................164.2 Compatible Cleaning Agents...................................................................................164.3 Maintenance Schedule.............................................................................................174.4 Electrode Replacement.............................................................................................174.5 RNase Decontamination..........................................................................................18
Section 5 Troubleshooting..........................................................................................19Section 6 Ordering Information...............................................................................20
6.1 Sub-Cell Model 96 and 192 Systems.......................................................................206.2 Sub-Cell Model 96 and 192 System accessories.....................................................206.3 Related Bio-Rad Products........................................................................................21
Section 7 References...................................................................................................25
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