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Table Of Contents

A.2.1 Ionic Exchange Chromatography
A.2.2 Size-Exclusion Chromatography
A.2.3 Affinity Chromatography
Gel-Filtration Chromatography
Affinity Chromatography
A.2.4 Purification of Recombinant Proteins
A.2.5 Commercially Pre-packed Column Kits
B.1.2 Colorimetric Assays
B.2 Commercial Protein Estimation Kits
B.5 Gel Staining
B.5.1 Coomassie Blue Staining
B.5.2 Silver Staining
B.6 Western Blotting
B.7 Immunoprecipitations
Lipid Analysis
C.1 Lipid Extraction
C.1.2 Folch Extraction
C.1.5 Hexane Extraction for Neutral Lipids
C.1.6 Glycolipid Extraction
C.2 Thin Layer Chromatography
Mammalian Cell Culture
D.1 Primary Culture
D.1.1 Primary Cell Isolation and Culturing
D.1.2 Splenocyte isolation
D.2 Tissue Culture
D.2.1 Adherent Cells
D.2.2 Suspension Cells
D.2.3 Cell Maintenance
D.3 Cell Count
D.4 MammalianTransfection
D.5 Calcium Phosphate Co-Precipitation
D.6 Electroporation Protocol
D.7 LipofectamineTransfection Protocol
E.1 A typical light microscope
E.1 Poly-L-Lysine Immobilisation
E.3 Immunohistochemistry
F.1 Enzyme Assays
F.1.1 Enzyme Kinetics: Basics
F.1.2 Lipid Kinase Assay
F.1.3 Protein Kinase Assay
F.1.4 ProteinTyrosine Phosphatase Assay
F.1.5 Alkaline Phosphatase Assay
F.1.6 Caspase Assay
F.2 Functional Assays
F.2.1 Apoptosis Assay
F.2.3 Chemotaxis Assay
Suggested Readings
Appendix 1 Safety Issues
Appendix 2 Buffer Chart
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A Manual for Biochemistry Protocols

A Manual for Biochemistry Protocols



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Published by: server on Jan 07, 2010
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