MLAB 2401 - Clinical Chemistry Lab Manual
UNIT: Electromagnetic Radiation andPhotometric Equipment
To review the theory of electromagnetic radiation and the principle and use of common laboratoryinstruments using electromagnetic radiation.
Upon completion of this exercise, the student will be able to:1.State the wave and particle theory of electromagnetic radiation.2.Know what colors correspond to visible wavelengths.3.Identify the regions of the electromagnetic spectrum occupied by gamma, x-rays, UV, visible,IR, and microwaves indicating relative
.4.Know basic principles of how instruments determine results.5.List and compare the principles/applications of different photometric equipment.
1.Properties of light and radiant energy.
behaves as if it has electric and magnetic fields and is found as
of energy (photons) traveling in
(particle and wave theories). The moreenergy contained, the more frequent the wave and therefore, the shorter the wavelength.
are measured in nanometers (
) or 10 meters.
2.Interactions of light and matter. A.
– (absorption spectroscopy – UV, visible, infra-red, atomic)When an atom, ion, or molecule absorbs a photon, the additional energy results in analteration of state (it becomes excited). Depending on the individual “species,” this maymean that a valence electron has been put into a higher energy level, or that thevibration or rotation of covalent bonds of the molecule have been changed.In order for a ray of radiation to be
it must:1.have the same frequency of the rotational or vibrational frequency in themolecules it strikes, and;2.be able to give up energy to the molecule it strikes.