in 50% alcohol and deposited, respectively in theHerbarium of the Institute of Botany and BotanicalGarden ‘‘Jevremovac’’, Faculty of Biology, Universityof Belgrade (BEOU) and Herbarium of the Institute of Botany, Faculty of Pharmacy, University of Belgrade(HFF).
oucher specimens:1. Budva (Montenegro – Eumediterranean): rockyvegetation (class.
), at the alti-tude of 40m (
Lakusˇ ic´ , D
25 May 1995, HFF –
Fix.no. Bu 01-02
).2. Lus ˇtica – Stari Kras ˇic ´i (Montenegro – Eumediterra-nean): maquis (class.
), limestone, atthe altitude of 80m (
Lakusˇ ic´ , D. & B. 2225/96,
27July 1996, BEOU, HFF –
).3. Peloponnesus (Greece, Peloponnesus – Eumeditera-nean: maquis (
Stevanovic´ , V.
, May 1995, BEOU –
).4. Canyon Cijevna, village Dinos ˇa (Montenegro – sub-Mediterranean): rocky vegetation (class.
), limestone (
Lakusˇ ic´ , B., Jancˇ ic´ , R., Slav-kovska, V.,
09 July 1997, HFF –
).5. Canyon of Morac ˇa, Dromir (Montenegro – sub-Mediterranean): rocks above the river Morac ˇa (class.
Lakusˇ ic´ , B., Slavkovska, V.,Jancˇ ic´ , R.
12 July 1997, HFF –
).Anatomical analyses of leaves and stems were doneon permanent slides, prepared by the standard methodfor light microscopy. Cross-sections of the leaves (150samples) and stems (50 samples) were cut on a Reichertsliding microtome (up to 10
m thick). The sections werecleared in Parazone and thoroughly washed beforestaining in safranin (1% w/v in 50% ethanol) and alcianblue (1% w/v, aqueous).Epidermal peels (150 samples), for surface structuresand stomata analyses, were prepared using Jeffrey’ssolution (10% nitric acid and 10% chromic acid, 1: 1)and stained in safranin and alcian blue. All slides weremounted in Canada balsam after dehydration.Density and type of the leaf and stem hairs, as well asthe paradermal aspect of epidermal cells, were alsostudied with SEM (JOEL JSM-6460), for which thesamples were covered by gold.All morpho-anatomical measurements were donewith the Image Analyzer System Ozaria 2001 and thedata processed in the statistical package Statistica 4.5for Windows. For each of the quantitative characters, 30leaf samples and 10 stem samples were obtained fromdifferent individuals belonging to each of the ﬁvepopulations analyzed.Twenty-two quantitative characters of the statisticalanalysis were grouped in three categories: I, Leaf anatomy characters (13); II, Leaf shape characters (4)and III, Stem anatomy characters (5).I.
Leaf anatomy characters
: (1) Height of adaxialepidermal cells; (2) thickness of palisade tissue; (3)thickness of spongy tissue; (4) height of abaxialepidermal cells; (5) number of palisade layers; (6)surface area of adaxial epidermal cells; (7) surfacearea of abaxial epidermal cells; (8) surface area of abaxial stomata; (9) number of abaxial stomata;(10) number of adaxial glandular hairs; (11)number of abaxial glandular hairs; (12) number of adaxial non-glandular hairs; (13) number of abaxialnon-glandular hair.II.
Leaf shape characters
: (14) Leaf length; (15)distance between the largest leaf width point andthe leaf top; (16) the largest width of the leaf; (17)leaf surface area.III.
Stem anatomy characters
: (18) Stem diameter; (19)stem diagonal; (20) stem cortex thickness; (21)thickness of the stem vascular cylinder; (22) stempith diameter.
For each of the quantitative characters a univariatestatistic analysis was done on the basis of the followingparameters: average value, minimum, maximum, stan-dard deviation and standard error. The signiﬁcance of differences between the populations studied was estab-lished by multivariate analyses of variances (MANO-VA). The general structure of the sample variabilitywere established by Principal Component Analysis(PCA). For checking the hypothesis that the analyzedsample was composed of discrete groups, which aremorphologically differentiated one from the other, aDiscriminant Component Analysis (DCA) was done.Overall differences between the compared groups arepresented by Mahalanobius’ distances, which are usedfor clustering on the basis of UPGMA method.
Leaf shape and anatomy
The leaves of
are elliptically oval, being thewidest at the basal part, and rounded at the tip. The leaf margin is obtusely dentate. The leaf stalk is long. Itshould be pointed out that the leaf shape was always thesame in all the populations studied.In general, in all the populations studied the leaf length was between 12 and 27mm, whereas the leaf width ranged between 11 and 21mm. The leaf surfacearea varied between 80 and 370mm
.The leaf indumentum of all the plants studiedwas composed of glandular and non-glandular hairs
ARTICLE IN PRESS
B. Lakus ˇic ´et al. / Flora 201 (2006) 108–119110