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PROYECTO_BAGAZO DE CAÑA

PROYECTO_BAGAZO DE CAÑA

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Published by: bioalimentos on Jan 30, 2010
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UNIVERSIDAD CENTRAL DELECUADOR
FACULTAD DE CIENCIAS QUIMICAS
ESCUELA DE BIOQUIMICA Y FARMACIA
QUIMICA DE ALIMENTOS
MICROBIOLOGIAINDUSTRIAL
TEMA:
AISLAMIENTO, SELECCI\u00d3N Y PRESERVACI\u00d3N DE CEPAS
LEVADURIFORMESY F\u00daNGICASDEGRADADORASDEL
BAGAZO DE CA\u00d1A DEAZ\u00daCAR
Integrantes:
Arboleda Marcelo
C\u00f3ndor Bel\u00e9n
Cueva Nathaly
G\u00f3ngora Estefan\u00eda
Curso:
9\u00ba ALIMENTOS
2
INDICE

1 TEMA:..........................................................................................................................................4 2 PROBLEMA..................................................................................................................................4 3 ANTECEDENTES...........................................................................................................................4 4 JUSTIFICACION.............................................................................................................................5 5 HIPOTESIS....................................................................................................................................5 6 OBJETIVOS...................................................................................................................................5

6.1 OBJETIVO GENERAL.............................................................................................................5
6.2 OBJETIVOS ESPECIFICOS......................................................................................................5
7 MARCO TEORICO.........................................................................................................................6
7.1 Bagazo de ca\u00f1a de az\u00facar...................................................................................................6
7.1.1 Propiedades f\u00edsicas y qu\u00edmicas del bagazo.................................................................. 6
7.1.2 Componentes principales de la materia prima.............................................................. 7
7.2 Empleo de substrato org\u00e1nico por los microorganismos...................................................9
7.3 Organismos degradadores de lignina y mecanismo de biodegradaci\u00f3n..........................10
7.3.1 Levaduras degradadoras de lignina............................................................................ 11
8 MARCO METODOLOGICO..........................................................................................................12
8.1 METODOS GENERALES DE ANALISIS.................................................................................12
8.1.1 Muestreo......................................................................................................................12
8.1.2 Preparaci\u00f3n de la muestra.......................................................................................... 12
8.2 METODOS ESPECIFICOS DE ANALISIS................................................................................12

8.2.1 Dise\u00f1o del medio de cultivo......................................................................................... 12 8.2.2 AISLAMIENTO DE LEVADURAS Y HONGOS DEGRADADORES DE LIGNINA...... 13 8.2.3 PURIFICACION...........................................................................................................13 8.2.4 IDENTIFICACION........................................................................................................13 8.2.5 SELECCI\u00d3N DE LOS MICROORGANISMOS LEVADURIFORMES......................... 13

8.2.5.1
Curva de crecimiento y evaluaci\u00f3n de la capacidad degradadora de lignina.... 14
3
8.2.6 PRESERVACI\u00d3N........................................................................................................15
9 FACTIBILIDAD............................................................................................................................15
10
CRONOGRAMA...................................................................................................................... 15
11
BIBLIOGRAF\u00cdA........................................................................................................................ 16
12
ANEXOS................................................................................................................................. 18
12.1 Composici\u00f3n de medios de cultivo...................................................................................18

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