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DNA Basics

DNA Basics

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Published by daotan88

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Published by: daotan88 on Feb 03, 2010
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09/20/2011

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Using molecular marker technology instudies on plant genetic diversity
DNA-based technologies
DNA basics
Copyright: IPGRI and Cornell University, 2003 DNA basics 1
 
Copyright: IPGRI and Cornell University, 2003
 
DNA basics 2
2
Contents
!
The DNA molecule:
Structure and featuresReplicationSqueezing into the chromosomeSequence organizationCytoplasmic DNA
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DNA technology
Restriction enzymesNucleic acid electrophoresisDNA polymorphism
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DNA isolation procedures in pictures
 
Copyright: IPGRI and Cornell University, 2003
 
DNA basics 3
3
DNA and RNA are moleculesmade up of strings of nucleotidesA nucleotide consists of:
A pentose sugarA phosphate groupA nitrogenous base
The DNA molecule: structure and features
The building blocks of deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) arenucleotides. A nucleotide consists of:
·
A pentose sugar; in DNA, it is deoxyribose, and, in RNA, it is ribose
·
A phosphate group
·
A nitrogenous base, which can be a:
Purine base—adenine (A), guanine (G).
Pyrimidine base—cytosine (C), thymine (T). In RNA, uracil (U) replacesthymineThe DNA molecule comprises a chain built from four simple building blocks (A, G, C andT) that are assembled to form a double helix. The helix consists of two strands, each witha sugar-phosphate backbone, held together by a weak hydrogen bond between the basesadenine-thymine (two hydrogen bonds) and cytosine-guanine (three hydrogen bonds).The shapes of A and T, and of C and G are ‘complementary’ and form the reason whyDNA may copy itself. Two chains of backbones and bases running in opposite directions(antiparallel) form the double helical structure. The order or ‘sequence’ of these basesalong the chain forms the genetic code that carries the precise genetic instructions forthe organism to function.

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