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Reverse Phase Chromatography

Reverse Phase Chromatography

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Published by gvikrant

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Categories:Types, Research, Science
Published by: gvikrant on Feb 03, 2010
Copyright:Attribution Non-commercial

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05/11/2014

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Reversed PhaseChromatography
Principles and Methods
18-1134-16Edition AA
 
Reversed PhaseChromatography
 
Contents
1.Introduction..............................................................................................5Theory of reversed phase chromatography...............................................6The matrix................................................................................................9The ligands.............................................................................................11Resolution in reversed phase chromatography........................................13Resolution..............................................................................................13Capacity factor.......................................................................................14Efficiency................................................................................................15Selectivity................................................................................................17Binding capacity.....................................................................................18Critical parameters in reversed phase chromatography...........................19Column length...................................................................................19Flow rate............................................................................................19Temperature.......................................................................................20Mobile phase.....................................................................................20Organic solvent..................................................................................20Ion suppression..................................................................................21Ion pairing agents..............................................................................22Gradient elution.................................................................................23Mode of use............................................................................................24Desalting............................................................................................24High resolution separations...............................................................25Large scale preparative purification...................................................25Stages in a purification scheme..........................................................26Capture..............................................................................................26Intermediate stages.............................................................................27Polishing............................................................................................272.Product Guide.........................................................................................29SOURCE™ RPC.....................................................................................30Product description............................................................................30High chemical stability......................................................................32Excellent flow/pressure characteristics................................................34High capacity.....................................................................................35Availability........................................................................................36µRPC C2/C18.........................................................................................37Product description............................................................................37Chemical and physical stability..........................................................38Flow/pressure characteristics..............................................................38Capacity............................................................................................38Availability........................................................................................39Sephasil™ Protein/Sephasil Peptide.........................................................39Product description............................................................................39Chemical and physical stability..........................................................40Flow/pressure characteristics..............................................................40Availability........................................................................................40

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