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respirometric tests and results (ülkü pak)

respirometric tests and results (ülkü pak)

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Published by ulku
Firstly, Respirometric test was done with only peptone to determine response of the biomass. Secondly, Respirometric test was done with peptone and K-Acid mixture to observe the respirometry under inhibitory conditions.
Firstly, Respirometric test was done with only peptone to determine response of the biomass. Secondly, Respirometric test was done with peptone and K-Acid mixture to observe the respirometry under inhibitory conditions.

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Published by: ulku on Feb 18, 2010
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01/02/2013

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SLUDGE:
Municipal Sludge: Semi-liquid residue remaining from the treatment of municipal water andwastewater.[1]Municipal sludges are the natural end products of a microbial food chain in the wastewater treatment process. Microbes feed on organic components of waste until they can no longer derive energy from it
.
Sludge is produced at two steps in the process of treating the effluent. Primary sludge isrecovered by the first stage of the processing at the primary clarifier. Primary clarification isusually carried out by sedimentation, but can also be performed by dissolved air flotation. Insedimentation, the wastewater to be treated is pumped into large settling tanks, with the solids being removed from the tank bottom.These solids can range from 1.5% to 6.5% depending on the characteristics of the material.The overflow, or clarified water, is passed on to the secondary treatment. Secondary treatmentis usually a biological process in which micro-organisms convert the waste to carbon dioxideand water while consuming oxygen. The resulting solids are then removed throughclarification as in the primary treatment. The resulting sludge is then mixed with the primarysludge prior to dewatering and disposal. In general, primary sludges are easier to dewater thanthe biological sludges resulting from the second stage.[2]
Figure 1. Wastewater Treatment
1
 
Typical chemical composition of sludge was given below:
 Total Dry Solids (TS), %2-8Volatile Solids (% of TS)60-80Protein (% of TS)20-30Nitrogen (% of TS)1,5-4Phosphorus (% of TS)0,8-2,8Potash (% of TS)0-1Cellulose (% of TS)8-15Iron2-4Silica (% of TS)15-20pH5-8Alkalinity (mgCaCO
3
/L)500-1500Organic Acids (mg Hac/L)200-2000Energy Content Btu/lb10000-12500 Table 1. Typical chemical composition of sludge[3]
*The municipal sludge, which was used biomass for experiment, was taken fromPaşaköy WWTP.
AİM of The STUDY
Firstly, Respirometric test was done with only peptone to determine response of the biomass.Secondly, Respirometric test was done with peptone and K-Acid mixture to observe therespirometry under inhibitory conditions.
Materials and Reagents
1)
Lab-scale activated sludge reactor: 4 Liter reactor was chosen and set up for acclimation of the biomass.
2)
Peptone mixture (OECD) solution: (15000 mg COD/L) Some compounds were used to prepare OECD solution. To prepare 2 L OECD solution, which compounds were usedand how much concentrate of these compounds were consumed ,were given below:
 
Compound Concentration (g/2L)
2
 
Peptone32 gMeat Extract22 gUrea6 g NaCl1,4 gCaCl
2
.2H
2
O0,8 gMgSO
4
.7H
2
O0,4 g
2
HPO
4
5,6 gTable 2. Compounds and amounts of peptone mixture2L beaker was used to mix these compounds with distilled water on a heater. 750 mL distilledwater was added to beaker. Magnetic stirrer was put on beaker. Beaker put on the heater.Compounds were put in beaker 
 
successively and stirred. When homogeneous mixture wasobtanied dilute the solution to 2L with distilled water.
3)
K-Acid Solution: 363g/L feed concentration was used to prepare K-Acid solution.
4)
Macro and micronutrients (Sol A , Sol B)To prepare Sol A, required compounds and amounts of compounds were given below:CompoundFeed Concentration (g/2L) NH
4
Cl*120 gKH
2
PO
4
160 g
2
HPO
4
320 gTable 3. Compounds and Feed concentrations of Sol A
 NH
4
Cl was not added to mixture, because there was urea in peptone solution.2L beaker was used to mix these compounds with distilled water on a heater. 750 mL distilledwater was added to beaker. Magnetic stirrer was put on beaker. Beaker put on the heater.Compounds were put in beaker 
 
successively and stirred. When homogeneous mixture wasobtained dilute the solution to 2L with distilled water.To prepare Sol B, required compounds and amounts of compounds were given below:CompoundFeed Concentration (g/2L)
3

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