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Dinh Luong Protein

Dinh Luong Protein

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Published by Rô Ron

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Published by: Rô Ron on Mar 19, 2010
Copyright:Attribution Non-commercial

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03/19/2010

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§Þnh l-îng protein vµ c¸c ph-¬ng ph¸p x¸c ®Þnh
1) X¸c ®Þnh hµm l-îng protein trong mÉu2) Ph©n tÝch amino acid3) §¸nh dÊu phãng x¹ protein4) §o hµm l-îng protein tæng b»ng so mµu5) Gi¶i tr×nh tù aa (Edman degradation)
 
X
¸c ®Þnh dung dÞch protein:ViÖc quan träng trong qu¸ tr×nh tinh chÕ proteinlµ thu nhËn protein chóng ta ®· t¸ch. §iÒu ®ã cãthÓ thùc hiÖn ®-îc nhê:
Ho¹t tÝnh enzyme, nÕu nã lµ métenzyme.§¬n gi¶n lµ bÊt kú ph-¬ng ph¸p thuËn tiÖn nµo cñaqua tr×nh ®Þnh l-îng c¸c thµnh phÇn mong muèn,nh-
Electrophoresis.
 
X
¸c ®Þnh dung dÞch protein:
ViÖc quan träng thø hai ®Ó biÕt ®-îc tængl-îng protein cã trong ph©n ®o¹n mµ ë ®ãchóng ta ®· thu ®-îc enzyme cñam×nh/protein ®Æc tr-ng.

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