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Custom Applied Biosystems TaqMan microRNA assay-like U6B RT-qPCR assay

Custom Applied Biosystems TaqMan microRNA assay-like U6B RT-qPCR assay

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Published by drpatnaik
We designed, custom-ordered and tested a set of human U6B primer/probe oligonucleotides, and found them to be as good as Applied Biosystem's TaqMan U6B microRNA assay kit (actually, 2-3x more sensitive) yet upto ten times cheaper -- this document provides more details. Note that the probe can be used at 100 nM concentration with barely any negative effect.
We designed, custom-ordered and tested a set of human U6B primer/probe oligonucleotides, and found them to be as good as Applied Biosystem's TaqMan U6B microRNA assay kit (actually, 2-3x more sensitive) yet upto ten times cheaper -- this document provides more details. Note that the probe can be used at 100 nM concentration with barely any negative effect.

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Categories:Types, Research, Science
Published by: drpatnaik on Apr 15, 2010
Copyright:Attribution Non-commercial

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07/15/2012

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