The high pH eliminates hydrogen bonds between bases, making it easier to separate the strands of DNA.
How many different amino acids could theoretically be encoded by nucleic acids containing four differentnucleotides if (a) each nucleotide coded for one amino acid; (b) consecutive sequences of two nucleotidescoded for one amino acid; (c) consecutive sequences of three nucleotides coded for one amino acid; (d)consecutive sequences of four nucleotides coded for one amino acid?
The number of possible sequences of four different nucleotides is 4
is the number of nucleotidesin the sequence. Therefore, (a) 4
= 4, (b) 4
= 16, (c) 4
= 64, and (d) 4
The recognition sequence for the restriction enzyme
I is T
CGA. Indicate the products of the reactionof
I with the DNA sequence shown.5
-AC GT C GAA T C –3
-T G C AGC T T A G–5
Using the data in Table 3-2, identify restriction enzymes that (a) produce blunt ends; (b) recognize andcleave the same sequence (called
); (c) produce identical sticky ends.
Describe the outcome of a chain-terminator sequencing procedure in which (a) too little ddNTP is added;(b) too much ddNTP is added; (c) too few primers are present; (d) too many primers are present.
Newly synthesized chains would be terminated less frequently, so the bands representingtruncated fragments on the sequencing gel would appear faint.
Chain termination would occur more frequently, so longer fragments would be less abundant.
The amount of DNA synthesis would decrease and the resulting gel bands would appear faint.
Calculate the number of clones required to obtain with a probability of 0.99 a specific 5-kb fragmentfrom
genome contains 97,000 kb, so
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