Phe. Ala and Phe are both hydrophobic, but Phe is much larger and might not fit as well in Val's place.
Asp. Replacing a positively charged Lys residue with an oppositely charged Asp residue would bemore disruptive.
Glu. The amide-containing Asn would be a better substitute for Gln than the acidic Glu.
His. Pro's constrained geometry is best approximated by Gly, which lacks a side chain, rather than a residue with a bulkier side chain such as His.
Laboratory techniques for randomly linking together amino acids typically generate an insoluble polypeptide, yet a naturally occurring polypeptide of the same length is usually soluble. Explain.
A polypeptide synthesized in a living cell has a sequence that has been optimized by natural selection sothat it folds properly (with hydrophobic residues on the inside and polar residues on the outside). Therandom sequence of the synthetic peptide cannot direct a coherent folding process, so hydrophobic sidechains on different molecules aggregate, causing the polypeptide to precipitate from solution.
Given enough time, can all denatured proteins spontaneously renature?
Describe the intra- and intermolecular bonds or interactions that are broken or retained when collagen isheated to produce gelatin.
Hydrophobic effects, van der Waals interactions, and hydrogen bonds are destroyed during denaturation.Covalent cross-links are retained.
Under physiological conditions, polylysine assumes a random coil conformation. Under what conditionsmight it form an
At physiological pH, the positively charged Lys side chains repel each other. Increasing the pH abovethe p
(>10.5) would neutralize the side chains and allow an
helix to form.
It is often stated that proteins are quite large compared to the molecules they bind. However, whatconstitutes a large number depends on your point of view. Calculate the ratio of the volume of ahemoglobin molecule (65 kD) to that of the four O
molecules that it binds and the ratio of the volume of a typical office (4 × 4 × 3 m) to that of the typical (70-kg) office worker that occupies it. Assume thatthe molecular volumes of hemoglobin and O
are in equal proportions to their molecular masses and thatthe office worker has a density of 1.0 g/cm
. Compare these ratios. Is this the result you expected?
The molecular mass of O
is 32 D. Hence the ratio of the masses of hemoglobin and 4 O
, which is equalto the ratio of their volumes, is 65,000/(4 × 32) = 508. The 70-kg office worker has a volume of 70 kg ×
Problemshttp://edugen.wiley.com/edugen/courses/crs1900/rc/voet9301c06/dm9ld...3 of 44/17/2010 9:12 AM