3AHAMD SARKI GUMEL
To observe and learn the different reproductive tract of the mouse putting emphasis ontheir size and appearance when ovulated or the regions that have the sperm and theirposition for applying the knowledge in future IVF work.
To learn the techniques employed in storing the recovered sperm and Oocytes usingdifferent invitro culture media and the techniques of using CO2 incubator
To learn how to make invitro fertilization and incubate the fertilized oocytes using theCO2 incubator.
To learn the technique employed in embryo recovery from copulated female mouse
To observe any short comings during the process and make possible suggestions.
Materials and Methods:
Animal Samples: laboratory Male mouse and Superovulated female mice (12 hourspostovulation) sacrificed humanely
TYH (Toyodo Yokoyama Hoshi) medium, WM (Whitten Medium), HWM (Hepes bufferedWhitten medium),BSA (Bovine serum Albumen)
4, 5 forceps, 4 dissecting scissors, 6 dissecting scissors, dissecting board, aluminum foil,gauze, 4-well culture dish, glass micropipette, hand gloves, stereomicroscope
Methodology:1.0 Sperm Recovery:
We were provided by a matured male mouse thatspent some period isolated from females .We killed theanimal by cervical dislocation using edge of a rubber cup in the laboratory sink.The cup-edge was placed at the base of the skull, little pressure was applied while twisting theedge on the neck and pulling the tail backward, at start the mouse was struggling, then afterabout two minutes we observed the mouse to be like in a quite sleep, by then noticed that ithas been killed.