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Introduction in chromatography.ppt

Introduction in chromatography.ppt

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Published by: Habibaq on Apr 18, 2010
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05/31/2013

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An Introduction toChromatography
What IS chromatography?The separation of a mixture by distributionof its components between a mobile andstationary phase over time
 – mobile phase = solvent – stationary phase = column packing material
 
Chromatography
Chromatogram - Detector signal vs.retention time or volumetime or volume
DetectorSignal
12
 
Milestones in Chromatography
1903 Tswett - plant pigments separated on chalk columns1931 Lederer & Kuhn - LC of carotenoids1938 TLC and ion exchange1950 reverse phase LC1954 Martin & Synge (Nobel Prize)1959 Gel permeation1965 instrumental LC (Waters)

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