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Diabetes and Chromatography

Diabetes and Chromatography

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Published by exjupiter
February 11, 2010
www.iwanttobescientist.blogspot.com

DETECTION OF DIABETES USING CHROMATOGRAPHY TECHNIQUE

What is Diebetes?
Glucose is an important source of energy for the body. We obtain glucose either from the diet or from production by the liver. Liver stored or released glucose to maintain the optimum level of glucose in the blood. Insulin, a hormone synthesized by the pancreas, allows cells to utilize glucose, which reduces blood glucose levels. The more glucose in your blood, the more insulin the pancreas releases. Diabe
February 11, 2010
www.iwanttobescientist.blogspot.com

DETECTION OF DIABETES USING CHROMATOGRAPHY TECHNIQUE

What is Diebetes?
Glucose is an important source of energy for the body. We obtain glucose either from the diet or from production by the liver. Liver stored or released glucose to maintain the optimum level of glucose in the blood. Insulin, a hormone synthesized by the pancreas, allows cells to utilize glucose, which reduces blood glucose levels. The more glucose in your blood, the more insulin the pancreas releases. Diabe

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Published by: exjupiter on Apr 21, 2010
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February 11,2010DETECTION OF DIABETES USING CHROMATOGRAPHY TECHNIQUEFahmi 20072878241
What is Diebetes?
Glucose is an important source of energy for the body. We obtain glucose either from thediet or from production by the liver. Liver stored or released glucose to maintain theoptimum level of glucose in the blood. Insulin, a hormone synthesized by the pancreas,allows cells to utilize glucose, which reduces blood glucose levels. The more glucose in yourblood, the more insulin the pancreas releases. Diabetes is caused by an inability to properlyprocess glucose, resulting in chronic hyperglycemia. Chronic hyperglycemia results in long-term damage to various organs such as the kidneys, heart, and eyes
 
There are 3 primary types of diabetes. First is type 1 diabetes or can be called as insulin-dependent diabetes mellitus or juvenile-onset diabetes. It is mainly caused by autoimmune,genetic, and environmental factors and make or involve in the destruction of the insulin-producing cells in the pancreas. Patients require treatment with insulin to regulate bloodglucose back to normal.Second is type 2 diabetes or called adult-onset diabetes. Type 2 diabetes is cause by obesity,genetic factor and age. Person who is diagnosed as type 2 diabetes will develop insulinresistance and require increase insulin production and thus need insulin treatment. Type 2diabetes is the most common diabetes and type 3 diabetes is gestational diabetes whichdevelops during pregnancy.For diabetes patients, blood is use in order to detect or manage diabetes. Certain markersare important in determining amount of sugar in blood or glucose level. One of thoseimportant markers is Hemoglobin A1c (HbA1c) and by using certain tests HbA1c can bemeasured and reflect the blood sugar level.Formation of Insulin
 
February 11,2010DETECTION OF DIABETES USING CHROMATOGRAPHY TECHNIQUEFahmi 20072878242
Role of HbA1c in Diabetes Mellitus
HbA1c is a part of hemoglobin (Hb) and can best define as glucose bound to N terminal of valine of B chain. HbA1c plays important role in Diabetes mellitus (DM) patient. HbA1c isobtain from patients blood sample and tested with several laboratory tests such aschromatography, immunoassays, and electrophoresis in order to adjust insulin dosing,assess response to exercise and meals, and prevent hypoglycaemia
What is Hemoglobin A1c?
 Hemoglobin A1c (HbA1c), also known as glycosylated hemoglobin or glycohemoglobin, is ameasure of a patients blood glucose level. It measure in 2-3 months. HbA1c consist of haemoglobin A (HbA) and glucose. It forms slowly and nonenzymatically from hemoglobinand glucose. This mechanism leads to the Amadori effect and produced a stable productwhich is ketoamine that we usually know as glycosilated haemoglobin (HbA1c). It is formedwhen glucose in the blood binds irreversibly to hemoglobin to form a stable complex.
 
February 11,2010DETECTION OF DIABETES USING CHROMATOGRAPHY TECHNIQUEFahmi 20072878243
The hemoglobin A1 has three different subtractions which are A, B, and C. The bondbetween glucose and the c-subtraction of the HB A1 is more stable. Hence, it may gives abetter information about the average glucose levels during the last 2-3 months. Each HbA1ccan lasts up to 120 days in blood based on average of red blood cells lifespan. Thus HbA1c isonly eliminated when the red cells replace. HbA1c values are directly proportional to theconcentration of glucose over approximately 2-3 monthsThe Hb A1c measurement can be made any time during the day even if on fasting period ornot because obviously is independent of the glucose values in that moment. Using specificchromatography technique such as Boronate Affinity Chromatography, it can measureHbA1c values. This test is done after a blood sample is taken from venipuncture but it canalso be performed with a capilar blood sample the same way you do at home when youmake a blood glucose test and send it to laboratory. The frequency of this lab test will varydepending upon your type of diabetes and the individual needs of every person withdiabetes Insulin treated persons independently of their diabetes type will have their Hb A1cmeasured every 3 months. Some special cases as the pregnant diabetes women will do itevery monthAs we know the average span life of a red blood cell is 3 months and that is the reason whythis lab test provides us with information about the degree of diabetic control during thisperiod of time. An increase of 1% in the Hb A1c value shows an increase of 30 mgrs in theaverage glucose levels.
Ideal: < 6.5% Acceptable: 6.5 - 7.5% Poor: > 7.5%Source from: World Health Organization (WHO)

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