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IMVIC test

IMVIC test

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Published by: Laksilu Viduraga Peiris on May 01, 2010
Copyright:Attribution Non-commercial


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5.0 IMViC TestTitle
: IMViC Test
: 20.03.2010
Experiment No
5.1 Introduction
:There four test to differentiate between principle of microorganisms of enterobacteriaceae. This group can be found in the intestinal tract of human and lower mammals.This family includesa.Pathogens-
Salmonella, Shigella
 b.Occasional pathogens-
 Proteus, Kiebsiella
c.Normal Intestinal Flora-
 Escherichia, Enterobactor 
IMViC reactions are a set of four useful reactions that are commonly employed in theidentification of members of family enterobacteriaceae. The four reactions are: Indoletest, Methyl Red test, Voges Proskauer test and Citrate utilization test. The letter “i” isonly for rhyming purpose.This test uses Simmon's citrate agar to determine the ability of a microorganism to usecitrate as its sole carbon source. The citrate agar is green before inoculation, and turns blue as a positive test indicator.These IMViC tests are useful for differentiating the familyEnterobacteriaceae,especially when used alongside theUrease test.
Except for the lowercase “i”, which is added for ease of pronunciation, each of theletters in “IMViC” stands for one of these tests. “I” is for indole; “M” is for methylred; “V” is for Voges-Proskauer, and “C” is for citrate.
5.2 Indol Test5.2.1 Introduction
Some bacteria can produce indole from amino acid tryptophan using the enzymetyptophanase.Production of indole is detected using Ehrlich’s reagent or Kovac’s reagent. Indole reacts withthe aldehyde in the reagent to give a red color. An alcoholic layer concentrates the red color as a ring at the top.
5.2.2 Materials
Cultures: 24 to 48 hour bacterial cultures of 
 Escherichia coli
Staphylococcus aureus
Media: SIM agar/Peptone water 
Tubes with tryptone /peptone broth
Reagent: Kovac’s reagent(p-dimethyl amino benzaldehyde , HCl and butanol)
Equipment: Bunsen burner 
Inoculating loop
Glass ware marking pencil
5.2.3 Procedure
The tubes of Tryptone/Peptone were inoculated using sterile techniquewith given bacterial culture-
Staphylococcus aureus
One tube was served as control

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