Except for the lowercase “i”, which is added for ease of pronunciation, each of theletters in “IMViC” stands for one of these tests. “I” is for indole; “M” is for methylred; “V” is for Voges-Proskauer, and “C” is for citrate.
5.2 Indol Test5.2.1 Introduction
Some bacteria can produce indole from amino acid tryptophan using the enzymetyptophanase.Production of indole is detected using Ehrlich’s reagent or Kovac’s reagent. Indole reacts withthe aldehyde in the reagent to give a red color. An alcoholic layer concentrates the red color as a ring at the top.
Cultures: 24 to 48 hour bacterial cultures of
Media: SIM agar/Peptone water
Tubes with tryptone /peptone broth
Reagent: Kovac’s reagent(p-dimethyl amino benzaldehyde , HCl and butanol)
Equipment: Bunsen burner
Glass ware marking pencil
The tubes of Tryptone/Peptone were inoculated using sterile techniquewith given bacterial culture-
One tube was served as control