AP BIOLOGY LAB 4Study of Photosynthesis Rate in DifferentChloroplasts Suspensions under Different Light Conditions
During the process of this lab, the researcher and his lab partners determined therate of photosynthesis in isolated chloroplasts using the dye DPIP; learned how toseparate pigments and calculate their R values; described a technique used to determine photosynthetic rates; compared photosynthetic rates at different wavelengths of lightusing controlled experiments and explained why the rate of photosynthesis varies under different environmental conditions.The researcher and his lab mates did this by labeling 5 cuvettes 1-5, then coveredthe walls and bottom of cuvette 2 with foil and made a foil cap to cover the top. 1ml of phosphate buffer was added to each cuvette; 4ml of distilled water was added to c1, 3mlof the water in 2, 3, 4 along with 1ml of DPIP. In c5, 3ml plus 3 drops of water and 3mlof DPIP was added. The researcher and his lab mates then followed the instructionswithin the lab booklet to obtain suspensions for the chloroplasts.The results from our experiment indicated a major connection between lightexposed to live chloroplasts and light transparency through a cuvette (amount of DPIPreduced). All mixtures except the unboiled chloroplasts exposed to light remainedrelatively constant in light transmittance. This includes unboiled/dark, boiled light, andno chloroplast/light mixtures. The dark unboiled suspension did increase a small amountin light transmittance, but as cited as error, this was probably due to unwanted lightemission.
Experiment using the scientific method.
:In this lab you will:1. separate plant pigments using chromatography and2. measure the rate of photosynthesis in isolated chloroplasts using the dye DPIP.The transfer of electrons during the light-dependent reactions of photosynthesis reducesDPIP, changing it from blue to colorless.Before doing this lab you should understand:
how chromatography separates two or more compounds that are initially presentin a mixture;
the process of photosynthesis;
the function of plant pigments;
the relationship between light wavelength and photosynthetic rate; and
the relationship between light intensity and photosynthetic rate.