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Primer on molecular genetics

Primer on molecular genetics

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Published by: nanobiologist on Jun 08, 2008
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Primer on Molecular Genetics
 
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DOE Human Genome Program
Primer on Molecular Genetics
Date Published: June 1992U.S. Department of EnergyOffice of Energy ResearchOffice of Health and Environmental ResearchWashington, DC 20585The "Primer on Molecular Genetics" is taken from the June 1992 DOE
Human Genome 1991-92 Program Report 
. The primer is intended to be an introduction tobasic principles of molecular genetics pertaining to the genome project.Human Genome Management Information SystemOak Ridge National Laboratory1060 Commerce ParkOak Ridge, TN 37830Voice:
865
 /576-6669Fax:
865
 /574-9888E-mail: bkq@ornl.gov
 
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Contents
Primer onMolecularGenetics
Revised and expandedby Denise Casey(HGMIS) from theprimer contributed byCharles Cantor andSylvia Spengler(Lawrence BerkeleyLaboratory) andpublished in the
Human Genome 1989– 90 Program Report 
.
Introduction
.............................................................................................................5DNA...............................................................................................................................6Genes............................................................................................................................7Chromosomes...............................................................................................................8
Mapping and Sequencing the Human Genome
......................................10Mapping Strategies.....................................................................................................11Genetic Linkage Maps............................................................................................11Physical Maps.........................................................................................................13Low-Resolution Physical Mapping......................................................................14Chromosomal map.........................................................................................14cDNA map......................................................................................................14High-Resolution Physical Mapping.....................................................................14Macrorestriction maps: Top-down mapping...................................................16Contig maps: Bottom-up mapping..................................................................17Sequencing Technologies...........................................................................................18Current Sequencing Technologies.........................................................................23Sequencing Technologies Under Development.....................................................24Partial Sequencing to Facilitate Mapping, Gene Identification...............................24End Games: Completing Maps and Sequences; Finding Specific Genes..................25
Model Organism Research
..............................................................................27
Informatics: Data Collection and Interpretation
.....................................27Collecting and Storing Data........................................................................................27Interpreting Data.........................................................................................................28Mapping Databases....................................................................................................29Sequence Databases..................................................................................................29Nucleic Acids (DNA and RNA)................................................................................29Proteins..................................................................................................................30
Impact of the Human Genome Project
.......................................................30
Glossary
...
..............................................................................................................32

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