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ELISA 1

ELISA 1

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Published by obai abed
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Published by: obai abed on Jun 11, 2008
Copyright:Attribution Non-commercial

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11/24/2012

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Introduction
ELISA: is an abbreviation for enzymelinked immunosorbent assay
ELISA: is a biochemical technique usedmainly in immunology
to detect the presence of Ab or Ag in a sample
also used as a diagnostic tool in medicine andplant pathology
quality control check in various industries
 
 
General steps and components
The main steps and components involvedin ELISA running:
2.
Solid phase: represented by microtiter plate which must have certaincharacteristics like
high level of adsorption of proteinioussubstances
the wells are flat in shape
3.
Coating step: with Ag or Ab

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