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Metode Ekspresi Kloning Gen

Metode Ekspresi Kloning Gen

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DOWNLOAD : http://www.ziddu.com/download/10801444/METODEEKSPRESIKLONINGGEN.pdf.html
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Published by: Hendrik_Nurfitrianto on Jul 20, 2010
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05/26/2013

 
COC @QMNLOE]B]ON
[oo| `n` d`|o |mboe l`qmsb`eo|don {|s}d|}s lon h}nf{` }|oio fmn. D`|o {`oq}n|}d imi}bo` {|}l` bmc`e s`nk los` c`gbgf` igbmd}bms. \`|`d cmso| qolo qmnpmb`l`don odon md{qms`imndon qoso oeb` c`gbgf` igbmd}bms |mboe l`|oiq`bdon{|s}d|}s lon h}nf{` fmn. Cmslo{osdon qmnloqo| `n`" d`|o odon imicoeo{ qg`n`n`}n|}d imnl`{d}{`d{n im|glm md{qms`imn ponf qmn|`nf los` c`gbgf`igbmd}bms.D`|o odon imi}bo` qmicoeo{on `n` lmnfon |mn`d smxgb}{` kbgn`nf fmn.Coponfdon doi} {mgsonf oeb` fmnm|`do qolo |oe}n ?063. doi} `nf`nimnfoio|` {|s}d|}s lon h}nf{` fmn m}dos`g|`d qolo |`nfdo| igbmd}bms. Doi} odon|msdm{on lmnfon egsign qms|}ic}eon ion}{`o %fmn efE). Oqodoe donl}nfon}|oio los` fmn `n`7 Imnfoqo qsgig|gs |msb`eo| {mqms|` `|}7 Oqo qmson SNO qgb`imso{m qolo fmn7 Oqo ponf |msjol` qolo fmn j`do dgnl`{` cms}coe7.Qms|onpoonqms|onpoon |ms{mc}| |`lod odon c`o{ l`jovoc j`do |`lod imiqmbojos`fmn lmnfon jmbo{.Lmnfon imnp`{`qdon fmn o{`nf qolo cod|ms`" iodo cod|ms` |ms{mc}| loqo|imiqsgl}d{` conpod fmn cos} lmnfon cmn|}d ponf jmbo{. Eob `n` odon l`coeo{ cofo`iono dbgn fmn qolo cod|ms` lon m}dos`g|`d.
 
COC @@DOJ@ON Q][\ODO
IM\GLM MD[QSM[@ DBGN@NF FMNImnfoqo d`|o `nf`n imnfdbgn`nf fmn7 ]n|}d {mc}oe {mcoc ponf|msb}qodon imnlgsgnf }n|}d imi}bo` io|ms` `n`. Kbgn`nf |msio{}d }{oeoimiqsgl}d{` loboi j}iboe ponf b}o{ {m{}o` lmnfon hod|o sonfdo`on LNO ponf|mboe d`|o qmbojos` {mkoso |msqms`nk`. Lmi`d`on fmn lmnfon {mnl`s`npo ioiq}imnjol` qsgl}d cmseosfo los` kbgn`nf fmn. Kbgn`nf fmn ims}qodon jobon bo`n}n|}d imiqmsb}o{ j}iboe qsgl}d fmn. [oboe {o|} dm}n|}nfon }n|}d imnpmb`l`d`|}j}on `n`.J`do jobon imnff}nodon cod|ms` }n|}d imiqsgl}d{` qsgl}d qsg|m`n los`{mc}oe kbgn`nf fmn m}dos`g|`d de}{}{npo ponf bmc`e |`nff` |`nfdo|onnpo. LNOi}nfd`n odon cmdmsjo bmc`e co`d los` qolo {mc}oe fmn ponf l`qg|gnf {mkosobonf{}nf los` fmngi. Dosmno qob`nf conpod fmn m}dos`g|`d |`nff` cms`{` qmnfeoico| {me`nffo cod|ms` |`lod loqo| imnfeoloq` {mb{mb m}dos`g|`d |ms{mc}|.[mb{mb m}dos`g|`d `n` c`o{onpo l`|msjmioedon gbme qmnfeoico| qmnp}{}n qsmiSNO lon imig|gnf dmb}os {}{}non {`{onpo {mkoso cms{oio }n|}d imnp}{}niSNO lmvo{o. Lmi`d`on {mc}oe LNO ponf imnfdgq` {mc}oe iSNO ponf |mboe{`oq imi`nloe qmnfeoico| lon ioiq} imnfmd{qsm{`don {mkoso cmnos loboi {mb cod|ms`.
Xmk|gs md{qsm{`
Xmd|gs ponf d`|o i`b`d` }n|}d imnfoio|` {onfo| jo}e odon l`f}nodon}|oinopo loboi kbgn`nf bonfdoe qms|oio dm|`do qms|oio dob` d`|o imnfoi`b LNOlos` lboi lon imio{}ddon dm {mc}oe cod|ms` }n|}d imnloqo|do smqb`do{`. Qolo}i}inpo imsmdo cmdmsjo }n|}d |}j}on |ms{mc}|" {`oq |}ic}e loboi M. Kgb` lonimiqsgl}d{` eo{`b ponf |`nff` los` LNO smdgic`non. cmcmsoqo los` imsmdo cmdmsjo qolo xmk|gs md{qsm{` ponf loqo| imnfeo{`bdon qsgl}d qsg|m`n los` fmnponf l`dbgn`nf. Kgn|genpo q]K lon qC[ ponf |msbm|od qolo {`{`qon xmk|gs lonl`o|}s gbme qsgig|gs }|oio" cmdmsjo l` lomsoe dmb`qo|on kbgn`nf. J`do {mc}oeLNO l`{`{`qdon l` |miqo| ponf {oio lmnfon fmn Bok z" qmbmc}son qsg|m`n odonl`eo{`bdon. @n` odon imiq}npo` {mc}oe cof`on los` qsg|m`n cm|o fobod|g{`lo qolo
 
oi`ng |msode`s lmnfon qmnp}{}n qsg|m`n bo`n" l`|msjmioedon loboi {`{`qon LNO" qolo doscgd{`b |msode`s.Cofo`ionoq}n j`do d`|o imnf}|oiodon md{qsm{` |`nff` los` fmn kbgn`nfde}{}{npo xmk|gs md{qsm{` c`o{onpo odon cmdmsjo bmc`e co`d. Xmk|gs md{qsm{` cod|ms` imiq}npo` {`ho| 3 mbmimn ponf qo{|` cmdmsjo od|`h loboi md{qsm{` fmn" qmnfo|}s ponf d}o| lon s`cg{gi ponf l`foc}nf l` lomsoe `n`{`o{` dglgn O\F.[oboe {o|} qsgig|gs ponf d}o| oloboe qsgig|gs |sq %|sp|gqeon gqmsgn)|ms{}{}n lmnfon lo{os }n|}d cmcmsoqo xmk|gs md{qsm{`" |msio{}d q|sq. Xmk|gs ponf cmdmsjo imiq}npo` qsgig|gs |s`q|gqeon o|o}q}n v`bopoe gqmso|gs" |msio{}d lmnfon d}iq}bon cmscofo` v`bopoe s`cg{gi lon loqo| l`f}nodon {mkoso bonf{}nf}n|}d xmk|gs md{qsm{` lmnfon {`{`qon fmn v`bopoe s`cg{gi.Qmnpmcoc xmk|gs md{qsm{` c`o{onpo imnf}n|}nfdon }n|}d fmn ponfl`dbgn`nf lon imnoeon {oiqo` vod|}npo. [}o|} obo{on qsg|m`n m}dospg|`l`qsgl}d{` loboi j}iboe ponf cm{os qolo cod|ms` }n|}d imnfeoico| sok}n. So|oso|o qsg|m`n `n` c}don ims}qodon sok}n {mcmnosnpo " |m|oq` imsmdo loqo|imn`nfdo|don qmsb`nl}nfon imsmdo }n|}d `d}| {ms|o loboi qms|}ic}eon cod|ms`.Io{oboe bo`n j`do kbgn`nf fmn |msio{}d qmnfeoico| {mkoso |m|oq. Fmn cod|ms` cms{`ho| |`lod odon |}ic}e imnjol` dgn{mn|so{` ponf k}d}q cm{os }n|}imiqsgl}d{` j}iboe qsgl}d qsg|m`n ponf {`fn`h`don. [gb}{` }n|}d imnjofokbgn`nf fmn l`io|`don lmnfon imnmiqo|don qolo cos`{on qsgig|gs ponfimi}nfd`ndon.Qsgig|gs bok oloboe qmnpmcoc imnjol` b}o{" d`sod`so l`os|`don {oiqo`sonf{onfon ponf l`c}o| {mcofo` qmnpmcoc `{`qsgq`bfobod|g{`lm %@Q\F).Cofo`ionoq}n" imnoeon l`{mcocdon gbme qmnoeon bok ponf |`lod dgiqbm| %cgkgs)lon cmcmsoqo kbgn`nf fmn odon l`oio|` dmcmsoloonnpo. [oboe {o|} jobon dmb}os los` io{oboe `n` oloboe md{qsm{` fmn loboi qbo{i`l o|o} qmicovo bok `|} {mnl`s`%qmnpmcoc fmn). Dmbmc`eon qmnoeon l`qsgl}d{` {mcofo` xmk|gs ponf imnjofokbgn`nf fmn l`emn|`don {oiqo` vod|} ponf |mboe l`|mn|}don.[|so|mf` bo`n imnff}nodon qsgig|gs {miq`| ponf l`o|}s {mcofo` cof`onobho. Xmk|gs md{qsm{` lmnfon {p{|mi qsgig|gs ponf l`dbgn`nf loboi {mb `nl}d ponf imiq}npo` |miqmso|}s {mn{`|`xm |mseoloq fmn obho. [mb{mb `n` |msjofo loboi|miqso|}s ponf smbo|`h smnloe %;3
1
K)" qmnpmcoc h}nf{` lon md{qsm{` cmdmsjo .

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