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Chinees Study XMRV(1)

Chinees Study XMRV(1)

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Published by: elipoarch on Sep 13, 2010
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05/19/2012

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Failure to detect xenotropic murine leukaemia virus-related virus in Chinese
patients with chronic fatigue syndrome
Virology Journal2010,7:224 doi:10.1186/1743-422X-7-224

Ping Hong (Pinghong98@yahoo.com)
Jinming Li (ljm63hn@yahoo.com.cn)
Yongzhe Li (yongzhelipumc@yahoo.com.cn)

ISSN1743-422X

Article typeResearch
Submission date19 July 2010
Acceptance date13 September 2010
Publication date13 September 2010

Article URLhttp://www.virologyj.com/content/7/1/224
This peer-reviewed article was published immediately upon acceptance. It can be downloaded,

printed and distributed freely for any purposes (see copyright notice below).
Articles inVirology Journal are listed in PubMed and archived at PubMed Central.
For information about publishing your research inVirology Journal or any BioMed Central journal, go

to
http://www.virologyj.com/info/instructions/
For information about other BioMed Central publications go to
http://www.biomedcentral.com/
Virology Journal

© 2010 Honget al. , licensee BioMed Central Ltd.
This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0),
which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

1
Failure to detect Xenotropic murine leukaemia virus-related virus in Chinese
patients with chronic fatigue syndrome
Ping Hong1, 2, Jinming Li1, 2*, Yongzhe Li3*
1Graduate School, Peking Union Medical College, Chinese Academy of Medical
Sciences, Beijing, People’s Republic of China
2National Center for Clinical Laboratories, Beijing Hospital, Beijing, People’s
Republic of China
3Peking Union Medical College Hospital, Beijing, People’s Republic of China
*Corresponding author: Jinming Li; Yongzhe Li

E-mail addresses
Jinming Li: ljm63hn@yahoo.com.cn
Yongzhe Li: yongzhelipumc@yahoo.com.cn
Ping Hong: Pinghong98@yahoo.com

2
Abstract
Background: Recent controversy has surrounded the question of whether

xenotropic murine leukaemia virus-related virus (XMRV) contributes to the
pathogenesis of chronic fatigue syndrome (CFS). To investigate the question in a
Chinese population, 65 CFS patients and 85 blood donor controls were enrolled and
multiplex real-time PCR or reverse transcriptase PCR (RT-PCR) was developed to
analyze the XMRV infection status of the study participants. The assay was
standardized by constructing plasmid DNAs and armored RNAs as XMRV standards
and competitive internal controls (CICs), respectively.

Results: The sensitivities of the multiplex real-time PCR and RT-PCR assays were

20 copies/reaction and 10 IU/ml, respectively, with 100% specificity. The within-run
precision coefficient of variation (CV) ranged from 1.76% to 2.80% and 1.70% to
2.59%, while the between-run CV ranged from 1.07% to 2.56% and 1.06% to 2.74%.
XMRV was not detected in the 65 CFS patients and 65 normal individuals out of 85
controls.

Conclusions: This study failed to show XMRV in peripheral blood mononuclear

cells (PBMCs) and plasma of Chinese patients with CFS. The absence of XMRV
nucleic acids does not support an association between XMRV infection and the
development of CFS in Chinese.

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