ERC Starting Grant Interview,May 27 2009
Analysis of gene expression by RNA-Sequencing
Rickard Sandberg
Ludwig Institute of Cancer ResearchDepartment of Cell and Molecular BiologyKarolinska InstitutetStockholm, Sweden
Sunday, September 12, 2010
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Expressed Sequence Tags
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Traditional 3’UTR focused microarrays
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Exon and Tiling Arrays
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Deep Sequencing using Illumina/Solexa, SOLiD, (454)
Methods for global mRNA isoform pro
ling
Sunday, September 12, 2010
Isolate polyA+ RNA
mRNA-seq protocol
Wang et al. 2009 Nat Rev Gen
Sunday, September 12, 2010
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