Journal of Biopesticides 3(1 Special Issue) 394 - 399 (2010)Antimicrobial activity of silver nanoparticles
Biogenic silver nanoparticles by
and their promising antimicrobial activity
K. Govindaraju, S.Tamilselvan, V. Kiruthiga and G. Singaravelu
Nanotechnology is gaining tremendous impetus in the present century due to its capability of modulatingmetals into their nanosize. Research in nanotechnology highlights the possibility of green chemistry pathwaysto produce technologically important nanomaterials. This report focuses on the biological synthesis of silvernanoparticles using
and its antimicrobial activity. Characterization of newly synthesized silvernanoparticles was made using UV-vis spectroscopy, Fourier Transform Infrared (FTIR) spectroscopy, X-raydiffraction (XRD) and High Resolution Transmission Electron Microscope (HR-TEM) studies. Resistance toantimicrobial agents by pathogen has emerged in recent years and is a major health problem.
mediated silver nanoparticles showed high antimicrobial activity against bacterial and fungal pathogens. Ourresults suggest that
mediated silver nanoparticles could act as an effective antimicrobial agent andprove as an alternative for the development of new antimicrobial agents to combat resistance problem.
silver nanoparticles, antimicrobial assay
Nanotechnology refers broadly to a field of appliedscience and technology whose unifying theme is thecontrol of matter on the atomic and molecular scale (AnimaNanda and Saravanan, 2009). Metal nanoparticles havereceived considerable attention in recent years becauseof their unique properties and potential applications incatalysis (Kamat, 2002), plasmonics (Maier
., 2001),optoelectronics (Gracias
., 2002), biological sensor(Mirkin
., 1996; Han
2001) and pharmaceuticalapplications (Chan and Nie, 1998). Their performancedepends critically on their size, shape and composition.Chemical synthesis methods are available for the synthesisof metal nanoparticles, many of the reactants and startingmaterials used in these methods are toxic and potentiallyhazardous in concern with biological applications(Ankamwar
., 2005). Consequently, an array of biological synthesis protocols leading to the formationof nanostructures have been reported using bacteria(Kalimuthu
., 2008; Anima Nandha and Saravanan,2009), fungi (Bhainsa and Souza, 2006; Vigneshwaran
., 2006; Basavaraja
2009) and plants (Chandran
2009a and 2009b; Singaravelu
.,2009). In this context it is noteworthy to mention thatsynthesis of inorganic nanoparticles by biologicalsystems makes nanoparticles more biocompatible andenvironmentally benign. We have recently reported onthe biological synthesis of gold nanoparticles using aphytochemcial (20
-dihydroxyurs-12-en-28-oic acid) and their PTP 1B inhibitory activity and inanother attempt biological synthesis of silver, gold andAg shell-Au core nanoparticles using single cell protein
havebeen achieved (Singaravelu
., 2007). Keeping thebiological perspectives in mind, the results reported hereinencompass the biological synthesis of silver nanoparticlesand their antimicrobial activity.
MATERIALS AND METHODSBiosynthesis of silver nanoparticles
Silver nitrate was purchased from Qualigens FineChemicals, Mumbai, India.
leaves werecollected from Vellore zone, Tamilnadu, India. UV–visiblespectra were recorded on Shimadzu UV-1601spectrophotometer containing double beam in identicalcompartments each for reference and test solution fittedwith 1-cm path length quartz cuvettes. The FT-IR spectrawere recorded using Perkin-Elmer FT-IR spectro-photometer. The AgNO
solution was centrifuged at 9,000 rpm for 25 minindividually. The deposited residue was dried and grinnedwith KBr to obtain pellet for the purpose of FT-IR analysis.X-ray diffraction (XRD) measurements of the bioreduced
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