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Laboratory Procedure Protocols for Parasitological Sample Collection Processing

Laboratory Procedure Protocols for Parasitological Sample Collection Processing



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Laboratory Procedure Protocolsfor Parasitological Samplecollection Processing andExamination of Parasite Eggs.
Dr.Kedar KarkiParasitology unit, Tripureswor Central Veterinary Laboratory
To diagnose gastro-intestinal parasites of ruminants, theparasites or their eggs/larvae must be recovered fromthe digestive tract of the animal or from faecal material.These are subsequently identified and quantified. Thefollowing are the main tasks involved in this process:1.Collection of faecal samples2.Separation of eggs/larvae from faecal material, andtheir concentration3. Microscopical examination of prepared specimens4. Preparation of faecal cultures5 Isolation and identification of larvae from cultures
basic limitations of faecalexamination in the diagnosis of gastro-intestinal parasitism.
(a) The demonstration of parasite eggs or larvae in thefaeces provides positive evidence that an animal isinfected but does not indicate the degree of an infection.(b) The failure to demonstrate eggs or larvae does notnecessarily mean that no parasites are present; theymay be present in an immature stage or the test usedmay not be sufficiently sensitive.(c) There is generally no correlation between thenumbers of eggs/larvae per gram of faeces and thenumber of adult nematodes present in cattle. Anexception to this may occur in a primary infection inyoung grazing animals during their first exposure. Thereare some indications that the correlation is stronger insheep and goats with mixed infections.

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the matter was of g8t help..thank u sir
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