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AP Biology lab 5

AP Biology lab 5



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Published by: Brad Simpson on Sep 27, 2010
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5/7/10AP 5Abstract: The experiment consists of setting up a room temperature bath and a 10
C bath.Obtain 3 100-mL graduated cylinders. Fill the first with 50mL of water, drop 25germinating peas in the cylinder, and determine the amount of water that was displaced.The second cylinder needs to be filled with 50mL of water, drop 25 dried peas into it, anddetermine the amount of water that was displaced. The last cylinder needs 50mL of water and 25 dried peas and add enough glass beads to attain a volume equivalent to that of thegerminating peas. Now recreate all three cylinders for use in respirometer 4, 5, and 6.Obtain 6 vials with and attached stopper and pipette. Place a small piece of cotton in the bottom of each vial and moisten the cotton with 15% KOH. Place the first set of germinating peas, dry peas plus beads, and beads in vials 1, 2, and 3 respectively. Placethe second set of germinating peas, dry peas plus beads, and beads in vials 4, 5, and 6respectively. Insert all the stoppers to each vial, and place a weight on the end of eachvial. Make a sling with masking tape across both baths. This is used to keep the pipetteout of water during an equilibrium period of 7 minutes. Vials 1, 2, and 3 should rest inthe room-temperature bath and vials 4, 5, and 6 should rest in the 10
C bath. After theequilibrium period, immerse all vials into their respective baths for 0, 5, 10, 15, and 20minutes. Every 5 minutes for 20 minutes check the readings on the pipette of the waters position and record it in a table.To set up the baths, two tubs are needed and must be filled halfway with water.The 10
C bath needs ice added to it continuously to keep it at the right temperature. Use aglass thermometer to measure and keep the water at a continuous temperature. Setting therest of the experiment up requires 6 vials with an attached stopper and pipette. Usemasking tape to create a sling across each bath to keep the pipette’s out of the water untilequilibrium has been achieved. After about 7 minutes, remove the masking tape andimmerse the pipette in the baths.The results should show that the room temperature germinating peas have thefastest rate of photosynthesis. The rate is .040 of O2 per minute while the 10
C bathyielded a rate of .001 O2 per minute.Emphasis: Data collection and analysisObjectives:Before doing this lab, you should understand:
Respiration, dormancy, and germination;
How a respirometer works in terms of the gas laws;
The general processes of metabolism in living organisms; and
How the rate of cellular respiration relates to the amount of activity in a cell.After doing this lab, you should be able to:
Calculate the rate of cell respiration from experimental data;
Relate gas production to respiration rate;
Test the rate of cellular respiration in germinating versus nongerminated seeds ina controlled experiment; and
Test the effect of temperature on the rate of cell respiration in germinating versusnongerminated seeds in a controlled experiment.Problem: What variable increases the rate of photosynthesis?Data:
Beads AloneGerminating PeasDry Peas and BeadsTime(Min)Readingat timeXDiff.Readingat timeXDiff.CorrectedDiff.∆Readingat timeXDiff.CorrectedDiff.∆Initial-0
3.38 0 3.16 .19 .19 3.46 .01 .01
3.38 0 3.04 .31 .31 3.44 .03 .03
3.38 0 2.93 .42 .42 3.43 .04 .0
3.38 0 2.57 .78 .78 3.42 .05 .05
3.39 .01 3.20 .12 .11 3.40 0 .01
3.38 .02 3.11 .21 .19 3.40 0 .02
3.38 .02 3.00 .32 .30 3.39 .01 .01
3.38 .02 2.95 .37 .93 3.38 .02 0
1. In this activity you are investigating both the effect of germination versusnongermination and warm temperature versus cold temperature on respiration rate.Identify two hypotheses being tested in this activity.The rate of cellular respiration is higher in the germinating peas in cold than in the beads or non-germinating peas; the cooler temperature in the cold-water baths slows the process of cellular respiration in the both germinating and non-germinating peas.2. This activity uses a number of controls. Identify at least three of the controls,and describe the purpose of each.
The constant temperature in the water baths yielding stable readings, theunvarying volume of KOH from vial to vial leading to equal amounts of carbondioxide consumption, identical equilibration periods for all the respirometers, precise time intervals between measurements, and glass beads acting as a controlfor barometric pressure all served as controls.3. Graph the results from the corrected difference column for the germinating peas and the dry peas at both room temperature and at 10 degrees C.For this graph, you will need to determine the following
The independent variable: Time Interval (minutes)
The dependent variable: Oxygen Consumption Change
Rate of Oxygen Consumption with varying variables
Time Intervals (Minutes)
   O  x  y  g  e  n   C  o  n  s  u  m  p   t   i  o  n   C   h  a  n  g  e
Beads at roomtemperatureBeads at 10 degreecelsiusGerminating Peas aroom temperatureGerminating Peas a10 Degrees CelsiusDry Peas and Beadat room temperatur Dry Peas and Beadat 10 Degrees Celsi
Describe and explain the relationship between the amount of oxygenconsumed and time. There was a constant, gradual incline in the amount of oxygen consumed over precise passage of time5.ConditionCalculationsRate in mL O
/minuteGerminating Peas/10
(3.40-3.38)20 min. .001

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