(e) An egg count often refers to the total number of eggs of a mixture of species, which differ widely both in their biotic potential and theirpathogenicity.(f) Eggs may not be detected due to low numbers of them or to a low testsensitivity.
Collection of faecal samples
Faecal samples for parasitological examination should be collected from therectum of animal.If rectal samples cannot be obtained, fresh Faecal samples may be collectedfrom the pasture.Several samples should be collected. Samples should be dispatched as soon aspossible to a laboratory in suitable containers such as:· screw cap bottles· plastic containers with lids· disposable plastic sleeves/gloves used for collecting the samples· plastic bagsEach samples should be clearly labeled with animal identification ,date andplace of collection.Samples should be packed and dispatched in a cool box to avoid the eggsdeveloping and hatching. If prolonged transport time to a laboratory isexpected, the following may help to prevent the eggs developing and hatching.(a) Filling the container to capacity or tightening the sleeve/glove as close tothe faeces as possible. This is to exclude air from the container.(b) Add 3%
formal in to the faeces (5-20 ml, depending on the volume of faeces). This is to preserve parasite eggs. (N.B Formalin-fixed faeces cannot beused for faecal cultures.) When samples are received in the laboratory theyshould immediately be stored in the refrigerator (4 °C) until they areprocessed. Samples can be kept in the refrigerator for up to
weeks withoutsignificant changes in the egg counts and the morphology of eggs. SAMPLESSHOULD NEVER BE KEPT IN THE FREEZER.
Qualitative techniques for separating and concentratingeggs/larvae