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Table Of Contents

1.General principles
Introduction
2.Microbiological risk assessment
Specimens for which there is limited information
Risk assessment and genetically modified microorganisms
Code of practice
Laboratory design and facilities
Laboratory equipment
Health and medical surveillance
Training
Waste handling
Chemical, fire, electrical, radiation and equipment safety
1.Code of practice
5.The maximum containment laboratory – Biosafety Level 4
6.Laboratory animal facilities
Animal facility – Biosafety Level 1
Animal facility – Biosafety Level 2
Animal facility – Biosafety Level 3
Animal facility – Biosafety Level 4
Invertebrates
invertebrates
7.Guidelines for laboratory/facility commissioning
8.Guidelines for laboratory/facility certification
9.Laboratory biosecurity concepts
10.Biological safety cabinets
Class I biological safety cabinet
Class II biological safety cabinets
Class III biological safety cabinet
Biological safety cabinet air connections
Selection of a biological safety cabinet
Using biological safety cabinets in the laboratory
11.Safety equipment
Negative-pressure flexible-film isolators
Pipetting aids
pipetting aids
Homogenizers, shakers, blenders and sonicators
Disposable transfer loops
Microincinerators
Personal protective equipment and clothing
12.Laboratory techniques
Safe handling of specimens in the laboratory
Use of pipettes and pipetting aids
Avoiding the dispersal of infectious materials
Use of biological safety cabinets
Avoiding ingestion of infectious materials and contact with skin and eyes
Avoiding injection of infectious materials
Separation of serum
Use of centrifuges
Use of homogenizers, shakers, blenders and sonicators
Use of tissue grinders
Care and use of refrigerators and freezers
Opening of ampoules containing lyophilized infectious materials
Storage of ampoules containing infectious materials
Standard precautions with blood and other body fluids, tissues and excreta
Precautions with materials that may contain prions
13.Contingency plans and emergency procedures
Contingency plan
Emergency procedures for microbiological laboratories
14.Disinfection and sterilization
Definitions
Cleaning laboratory materials
Chemical germicides
Local environmental decontamination
Decontamination of biological safety cabinets
Hand-washing/hand decontamination
Heat disinfection and sterilization
Incineration
Disposal
15.Introduction to the transport of infectious substances
International transport regulations
The basic triple packaging system
Spill clean-up procedure
16.Biosafety and recombinant DNA technology
Biosafety considerations for biological expression systems
Biosafety considerations for expression vectors
Viral vectors for gene transfer
Transgenic and “knock-out” animals
Further considerations
17.Hazardous chemicals
Routes of exposure
Storage of chemicals
General rules regarding chemical incompatibilities
Toxic effects of chemicals
Explosive chemicals
Chemical spills
Ionizing radiation
19.The biosafety officer and biosafety committee
Biosafety officer
Biosafety committee
20.Safety for support staff
Engineering and building maintenance services
Cleaning (domestic) services
21.Training programmes
22.Safety checklist
Laboratory premises
Storage facilities
Sanitation and staff facilities
Heating and ventilation
Lighting
Services
Laboratory biosecurity
Fire prevention and fire protection
Flammable liquid storage
the flammable liquid store?
Personal protection
Health and safety of staff
Infectious materials
Chemicals and radioactive substances
References
Equipment that may create a hazard
Index
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Biosafety Manual

Biosafety Manual

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Published by pkmdibr

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Published by: pkmdibr on Oct 01, 2010
Copyright:Attribution Non-commercial

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11/02/2011

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