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Bread Board Experiment-protocol

Bread Board Experiment-protocol

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Published by Robin Lewis Cooper
Model of neurons in electrical terms. Hands on teaching. Used for Bio350 Animal physiology labs at the Univ of KY., Lexington, KY, USA. Dept of Biology.
Model of neurons in electrical terms. Hands on teaching. Used for Bio350 Animal physiology labs at the Univ of KY., Lexington, KY, USA. Dept of Biology.

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Published by: Robin Lewis Cooper on Oct 01, 2010
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11/26/2013

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Modeling biological membranes with circuit boards: Student laboratory exercisesbyMartha M. Robinson
1
, Jonathan M. Martin
1
, Harold L. Atwood
2
and R. L . Cooper 
11
Department of Biology, University of KY, Lexington, KY 40506-0225, USA;
2
Department of Physiology, University of Toronto,Toronto, Ontario, M5S 1A8 CanadaABSTRACT
This is a demonstration of how electrical models can be used to characterize biologicalmembranes. This exercise also introduces biophysical terminology used inelectrophysiology. The equipment used in this membrane model will also be used onlive preparations later this semester. Several properties of an isolated nerve cord areinvestigated including: nerve actions potentials, recruitment of neurons, andresponsiveness of the nerve cord to environmental factors.
1.1) Background
A fundamental knowledge of electrical circuits is a valuable tool for understanding and conceptualizing many aspects of physiological experimentation andtheory. This exercise is intended to acquaint you with some general principles involvingvoltage sources, electrical resistance, and electrical capacitance. These introductoryconcepts provide the background for understanding such phenomena as synaptictransmission and the spread of electrical signals along a nerve fiber.A word of caution: In standard everyday electrical circuits composed of metalwires and resistors, CURRENT is carried by electrons, which have a negative charge. Inbiological systems however, current is carried by IONS, which may have one or morepositive or negative charges. By convention, current flows from positive to negative(although, of course, electrons actually move the other way).The components of the circuit represent the electrical properties of living tissue, and insome cases, that of inanimate objects (i.e. electrodes) involved in its study.In working through the exercise, keep notes of results and calculations.
1.2) General Principles
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The basic properties of electrical circuits in which direct current flows are described byOhm’s LawV = IRWhere V = voltage in volts (V)I = current in amps (A)R = resistance in ohms (Ω)This law governs the potentials which are observed in nerve cells with electrodes. Wewill first demonstrate Ohm’s Law and its application in biological materials.
1.3) Materials
ItemQuantityBreadboard1Resistors: 330 K 1: 100 K 12: 33 K 6: 22 K 1: 10 K 6: 4.7 K 1: 2.2 K 1: 1.5 K 1: 1 K 1: 510 1: 330 1: 220 1: 150 1: 100 1Capacitors: 0.10 µF6: 10.0 µF6Wires12
2
 
PowerLab1PowerLab Output Leads1PowerLab Input Leads1Voltmeter (Current Capable)1Resistor Coding Key
 
1
st
Band – 1
st
Digit2
nd
Band – 2
nd
Digit3
rd
Band – Number of Zeros4
th
Band – Tolerance
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ColorDigit
Black0Brown1Red2Orange3Yellow4Green5Blue6Violet7Grey8White9Silver10% ToleranceGold5% Tolerance

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