Welcome to Scribd. Sign in or start your free trial to enjoy unlimited e-books, audiobooks & documents.Find out more
Download
Standard view
Full view
of .
Look up keyword
Like this
1Activity
0 of .
Results for:
No results containing your search query
P. 1
Group 5

Group 5

Ratings: (0)|Views: 63|Likes:
Published by pixholic

More info:

Published by: pixholic on Oct 21, 2010
Copyright:Attribution Non-commercial

Availability:

Read on Scribd mobile: iPhone, iPad and Android.
download as DOCX, PDF, TXT or read online from Scribd
See more
See less

05/21/2012

pdf

text

original

 
GROUP 5: 3GMT
Matawaran, Luis PaoloMercado, Coleen IrisMiranda, RenzMontalbo, CarloNavarro, FrancisOsea, Rachelle
Family Vibrionaceae:
I.
Vibrio spp.
General Characteristics:
y
 
Grows well at very high pH (8.5-9.8)
y
 
Rapidly killed by acid
y
 
Grows well at 37ºC
y
 
Ferments sucrose and mannose (cannot ferment arabinose and inositol)
y
 
Halophiles
y
 
Can grow on media containing 6% NaCl
Virulence Factors:
y
 
E
ntero toxin  cholera toxin or choleragen
y
 
A
bility to induce hypersecretion of electrolytes (Na
+
, K
+
, HCO
3-
)
Microscopic morphology:
y
 
Gram negative rods
y
 
Pleomorphic under suboptimal conditions
y
 
Comma shaped or curved bacilli (only in the initial gram stain of the clinical specimen)
y
 
Usually appear as small , straight rods (on prolonged cultivation)
y
 
A
sporogenous
y
 
0
5-
0
8 µm x 1.5-3.
0
µm
Colony morphology:
 
Vibrio cholorae
Large yellow colonies in TCBS, Opaque andgranular in transmitted light
 
Vibrio parahaemolyticus
Beta hemolytic in BAP, green colonies (TCBS)
Vibrio vulnificus
Blue-green colonies in TCBS
Vibrio alginolyticus
Large yellow colonies in TCBS
 
Culture Media:
y
 
SBA
(
S
heep
B
lood
A
gar)
y
 
Chocolate agar - large colonies that appear smooth, opaque and iridescent with greenish hue.
y
 
Mac -Conkey agar - pathogenic vibrios usually grow as nonlactose fermenters
y
 
Thiosulfate Citrate
B
ile
S
alts sucrose agar (TC
BS
) -
S
elective and differential. Differentiates sucrose-fermentingspecies (yellow colonies) from nonsucrose-fermenting species (green colonies)
B
iochemical Tests:
T
ES
T
S
 
GROUP 1 GROUP 2 GROUP 3 GROUP 4 GROUP 5 GROUP 6
V.choleraeV.mimicusV.metschniikov ii V.cincinnatiensi s V. hollisaeV.damsel aV. fluviali sV.alginolyticusV. parahaemolyticusV.vulnificusV.harvery 
Nutrientbroth w/oNacl + + - - - - - - - - -Nutrientbroth w/ 1%Nacl + + + + + + + + + + +Oxidase test - + + + + + + + +Nitratereductiontest - + + + + + + + +Myo-InositolFermentation V + - - - - - - -
A
rgininedihydrolase - + + - - - -Lysinedecarboxylase - V - + + + +Ornithinedecarboxylase -
Pathogenesis:
 
Most common in the clinical laboratory
1
.
 
V. cholerae (serogroups O
1
and non-O
1)
 2.
 
V. parahaemolyticus3.
 
V. vulnificus4.
 
V. alginolyticus
1)
Vibrio cholera
- Patients with infection experience a rice water stool sample. May experience:
 
Dehydration
 
Hypovolemic shock
 
Metablic acidosis
 
Rapid fluid & electrolyte loss
 
Death2)
V. parhaemolyticus
 
 
S
elf limited gastrointestinal diseases
 
Kanawaga phenomenon  hemolysin and virulence3)
V. vulnificus
 
 
Primary septicemia
 
W
ound infection4)
V. Alginolyticus
  least pathogenic
II.
Plesiomonas spp.
 
General Characteristics:
y
 
Gram-negative comma bacilli
y
 
Polarly flagellated pathogenic bacterium
y
 
Motile
y
 
Habitat is fresh water
y
 
Oxidase positive
y
 
Facultative anaerobe
y
 
Glucose fermenterVirulence Factors:
y
 
E
ndotoxin production
y
 
B
eta-hemolytic
y
 
Cholera-like enterotoxin production
y
 
Large plasmid for invasionColony Morphology
 
Grows readily on most routine media
 
S
hiny, opaque, slightly raised center, smooth
 
Late-lactose fermenters
 
Non-hemolytic
 
Grows on most media routinely used in the clinical laboratory
 
18-24 hours of incubation at 35°C, shiny, opaque, nonhemolytic colonies appear.
 
Can grow well on CIN (cefsulodin-irgasin-novobiocin) as opaque colonies with an opaque apron.Pathogenesis:1) Plesiomonas Intestinal
 
Gastroenteris
 
W
atery secretory diarrhea
 
Colitis2) Plesiomonas
E
xtraintestinal

You're Reading a Free Preview

Download
scribd
/*********** DO NOT ALTER ANYTHING BELOW THIS LINE ! ************/ var s_code=s.t();if(s_code)document.write(s_code)//-->