iMedPub Journals

TRANSLATIONAL BIOMEDICINE

2010Vol.1No. 3:3doi: 10:3823/416

Micronutrient Optimization Storage Trial

Using Customized Vitamin & Mineral Replacement Therapy Most 2010

1Presented at the 10

INDC – International Nutrition & Diagnostics Conerence 20102Address reprint requests and correspondence to Dr. Darrin L. Frye, MD, 8409 N. Military Trail, Suite 126, Palm Beach Gardens,FL 33410 or email: ryemedical@aol.com

Darrin L. Frye, MD, MPH ABAARM

Introduction

Ultimate health and maximal lie span requires metabolic har-mony and nutrient abundance.

In order or the body’s cellularmachinery to unction properly, an adequate amount o bio-available nutrients plus a myriad o other essential substratesmust always be available. Recommended Daily Allowance (RDA)amounts o vitamins and minerals do little to prevent the epi-demic o chronic degenerative diseases. RDA’s were frst intro-duced in the 1930’s or the purpose o reducing scurvy, rickets,and pellagra. Today, however, RDA’s are a conusing and mis-leading standard, causing many people to think that i theyconsume the RDA amounts o vitamins and minerals, they’redoing all that’s necessary to be healthy. This is clearly alse anddamaging to a misguided and suering population.

Air pollu-tion, contaminated sources o water, radiation, over-processedoods, depleted soils, toxic chemicals, and prescriptive drugseect nutrient needs making RDA suggestions and nutritionist’smeal plans woeully inadequate.Macronutrient and micronutrient defciencies are very commonin the general population – it is estimated that over 1 billion peo-ple worldwide are vitamin D defcient, or at least insucient.

Overall it has been established by the scientifc community thatapproximately 70 percent o the U.S. population is at risk orlong-term vitamin and mineral defciencies.

Some individual

Background:

Ultimate health and maximal lie span require metabolic harmony and nutrient abundance. Evidence sug-gests that metabolic damage occurs at intake levels between the level causing micronutrient defciency diseases andthe recommended dietary allowances (RDA). This may result in an increase in DNA damage, neuronal or mitochondrialdecay that could lead to accelerated aging, cancers, and degenerative diseases. The optimum amount o vitamins andminerals that are truly required is the amount that minimizes DNA damage and maximizes a healthy lie span, which ishigher than the amount to prevent acute disease. Maintaining healthy vitamin and mineral stores prevent disease andlimit morbidity rom them.

Methods:

We examined prospectively a cohort o active adults aged 34 to 73 (median 53.5)

years o age who were seenat a preventive medicine center in 2009 - 2010. These 10 adult men and women had a comprehensive medical history andbaseline and 6 month cellular blood testing utilizing SpectraCell Laboratory’s comprehensive micronutrient method. Theywere treated therapeutically using IVitaminScience’s (IVS) custom ormulation supplementation therapy or six months.Individual nutrient values at six months were compared against their baseline values. Thirteen individual vitamin and tenmineral components that were supplemented based on a proprietary IVitaminScience algorithm.

Findings:

Both men and women showed substantial overall improvement in their vitamins and mineral cellular storagebalance. Results o the testing revealed mean deviation rom ideal o 15, that decreased rom 7.18 (standard deviation 1.12)to 5.73 (standard deviation 2.09) a 20.55% improvement in just six months.

Conclusions:

In a society o nutrient depleted ood sources, and poor dietary selection practices, determination o in-dividual nutrient defciencies and repletion with specifc therapeutic doses o vitamins and minerals appears to havepromise in restoring and maintaining health. Further studies on vitamin and mineral supplementation targeting diseasespecifc states should be conducted using this model since treatment can be initiated precisely, and objective ollow updata can be combined and correlated with clinical fndings.

iMedPub Journals

TRANSLATIONAL BIOMEDICINE

2010Vol.1No. 3:3doi: 10:3823/416

nutrient levels will predictably low in patients who are fghtingactive disease due to increased demand, but coupled with ge-netic predisposition issues and a myriad o other issues, levelscan easily become pathological. Micronutrient defciencies areinsidious usually, and recommendations or vitamin and min-eral supplementation or the apparently healthy are oten dis-regarded as superuous. It is unlikely that anyone ever has su-fcient levels o essential micronutrients at all times to meet theunpredictable demands o the body deense systems. Intuitively,it might seem that a low single nutrient level would make littledierence in the whole scheme o lie. However, when just onecellular request goes unflled repercussions are elt throughoutthe entire body and can lead to a myriad o acute and chronicdisease states.Unortunately, body reserves o vitamins and minerals do nothave to be even “out o the reerence range” or “signifcantlylow” in order to cause problems.

“Evidence suggests that meta-bolic damage occurs at intake amounts between the level caus-ing micronutrient defciency diseases and the recommendeddietary allowances (RDA). This may result in an increase in DNAdamage, neuronal or mitochondrial decay that could lead to ac-celerated aging, cancers, and degenerative diseases.

Dr. BruceAmes, a respected biochemist rom the University o Caliornia,Berkeley, showed that low levels o micronutrients (vitaminsand minerals) at the cellular level may actively promote DNAand protein damage and cause disease to a larger extent thanis generally realized. The optimum amount o vitamins and min-erals that are truly required is the amount that minimizes DNAdamage and maximizes a healthy lie span, which is higher thanthe amount to prevent acute disease.

Dr. Ames suggests thatvitamins and minerals play a critical role as coactors in enzymereactions that protect genes rom mutations and repair genedamage. Thus, certain micronutrients can act prophylactically,and also therapeutically.Numerous clinical trials have evaluated the use o nutritionalsupplements such as beta-carotene, selenium, vitamin C andvitamin E in the prevention o coronary heart disease and strokeyielding conicting results (positive, neutral and negative). Inmany o these clinical trials there were enormous clinical designproblems, methodological aws, varied patient populations,variable doses and type o vitamin use, improper selection o vitamins used and many other issues that make the studies di-fcult to interpret.

The MOST study is the frst

to run the meth-odological gauntlet, solving the clinical design and therapeuticchallenges that others haven’t. This pilot study produced en-couraging and exciting results, and established the viability,and capabilities o this revolutionary research tool. The MOSTmethodology should be utilized to modernize recommendeddaily allowances, improve tolerance and saety data, promotenew ocus into micronutrient research studies, and become aormidable oe o chronic and acute disease.

Methods

We examined prospectively a cohort o active adults aged 34 to73

years (median 53.5) who were seen at a preventive medicinecenter late 2009 through early 2010. This pilot study group wasselected merely as the frst group o ten available or analysis. The group o adults, 6 men and 4 women were given a propri-etary IVitaminScience (IVS) comprehensive health history ques-tionnaire and then had their initial phlebotomy. Once their cel-lular laboratory results were completed, the data was entered,along with the nutrient history fndings into the IVS’s proprietaryalgorithm that created individual, custom, therapeutic ormulasor each participant. (See Table 1.) Thirteen individual vitamin and ten mineral components thatwere supplemented, each patient had at least 4 capsules per dayo micronutrients and were asked to take their vitamins in the AMand their minerals in the PM. Each patient was also asked to re-rain rom any other micronutrient supplement or the durationo the study. At the end o 6 months, a second phlebotomy wasperormed and the results were compared against their baseline.Blood samples were collected in two 10 ml heparinized tubesand shipped overnight to the testing acility. Upon receipt, pe-ripheral blood mononuclear cells (over 90% lymphocytes) wereisolated by Ficoll density gradient centriugation and washed toremove platelets. Lymphocytes (150,000 cells/ml) were addedto wells o microtiter plates with a series o over 60 dierentmodifcations o CFBI 1000 proprietary media, stimulated togrow by addition o phytohemagglutinin (PHA), and incubatedor our days. Tritiated thymidine was then added, and incuba-tion continued or another day. Cells were then harvested toretain DNA on flters, and a direct-read beta counter countedradioactivity. Results were expressed as a percentage o control(optimal) growth.

Results

This trial was not only a supplemental challenge study, but alsoan eciency study. The goals o the program were to replaceall nutrients to a reasonable abundance, without creating largeexcesses. Target abundance was determined to a cellular nu-merical value o 15 when compared against the arithmetic di-erence rom control to observed values, while defciencies weredefned as levels below 7. (See Table 1)