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r05312302 Basic Industrial Biotechnology

r05312302 Basic Industrial Biotechnology

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Published by Srinivasa Rao G

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Published by: Srinivasa Rao G on Jul 22, 2008
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Code No: R05312302
Set No. 1
III B.Tech I Semester Supplimentary Examinations, February 2008
BASIC INDUSTRIAL BIOTECHNOLOGY
(Bio-Technology)
Time: 3 hours
Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
\u22c6 \u22c6 \u22c6 \u22c6 \u22c6
1. Brie\ufb02y write a historical overview of industrial fermentation process.

[16] 2. Describe and discuss the characteristics of an ideal production medium? [4+12] 3. Describe the production of gluconic acid by submerged fermentation process . How

it is recovered . What are the uses of gluconic acid ?
[8+4+4]
4. Explain the recovery and puri\ufb01cation of vitamin B12 from the fermentation broth?
[8+8]
5. Write short notes with examples on:
(a) Constitutive enzymes.
(b) Inducible enzymes.
(c) Extracellular and enocellular enzymes.
[4+6+6]
6. Enumerate di\ufb00erent feed back controls existing in various microbial systems? [16]
7. Describe the details of plant growth harmone production?
[16]
8. Write short notes on:

(a) Di\ufb00erence between vaccine and antibody.
(b) Immune response and vaccines.
(c) Disease control by vaccination.

[4+6+6]
\u22c6 \u22c6 \u22c6 \u22c6 \u22c6
1 of 1
Code No: R05312302
Set No. 2
III B.Tech I Semester Supplimentary Examinations, February 2008
BASIC INDUSTRIAL BIOTECHNOLOGY
(Bio-Technology)
Time: 3 hours
Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
\u22c6 \u22c6 \u22c6 \u22c6 \u22c6
1. Brie\ufb02y write a historical overview of industrial fermentation process.
[16]
2. Explain the importance of the following in bioprocess:
(a) Bu\ufb00ers.
(b) Media economics.
(c) Physiological conditions of the fermentation process.
[5+5+6]
3. Write short notes on:

(a) Ratio of surface area to volume in citric acid production. (b) Various steps involved in citric acid downstream process. (c) Media composition for gluconic acid production.

[4+6+6]
4. Enumerate the role of precursors and inducers in secondary metabolite production?
[16]
5. Name di\ufb00erent industrially useful enzymes, source of organisms and growth condi-
tions?
[6+6+4]
6. Explain phenomena of protoplast fusion? Give a detailed account of the techniques
of protoplst fusion?
[8+8]
7. Discuss the following:
(a) Advantages of natural preservatives over chemical preservatives.
(b) Somoclonal variations versus product production.
[8+8]
8. Write brie\ufb02y note on:

(a) Chemical properties of interferons.
(b) Functional speci\ufb01city of interferons.
(c) Uses of interferons.

[6+5+5]
\u22c6 \u22c6 \u22c6 \u22c6 \u22c6
1 of 1
Code No: R05312302
Set No. 3
III B.Tech I Semester Supplimentary Examinations, February 2008
BASIC INDUSTRIAL BIOTECHNOLOGY
(Bio-Technology)
Time: 3 hours
Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
\u22c6 \u22c6 \u22c6 \u22c6 \u22c6
1. Brie\ufb02y write a historical overview of industrial fermentation process.
[16]
2. Give detailed account of principles of isolation of industrially important microor-
ganisms?
[16]
3. (a) De\ufb01ne primary and secondary metabolites.
[4+4]
(b) Give three examples of each describing the outlines of the metabolic route. [8]
4. Enumerate the role of precursors and inducers in secondary metabolite production?
[16]
5. Describe the various steps involved in insulin production by recombinant microbial
species?
[16]
6. Write short notes:
(a) Various steps involved in auxotroph selection.
(b) Importance of metabolic \ufb02ow redirection in product production.
[8+8]
7. How biotechnology can be bene\ufb01cial for the conversion of vegetable oils to value
added products?
[16]
8. Describe the bacterial signi\ufb01cance of viral reproduction in improving vaccine pro-
duction?
[16]
\u22c6 \u22c6 \u22c6 \u22c6 \u22c6
1 of 1

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