Fluorescent Protein Bicistronic Expression System IRES

Box 1 | Product List Fluorescent Protein Vectors

Vector Name pmWasabi-IRES pmTFP1-IRES pLanRFP-IRES pLanYFP-IRES Cat. Number ABP-FP-WIRES ABP-FP-TIRES ABP-FP-RIRES ABP-FP-YIRES

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pmWasabi-IRES (ABP-FP-WIRES

CMV Promoter ........ 1-589 Multiple Cloning Sites ........ 591-652 IRES ........ 653-1243 mWasabi GFP ........ 1244-1954 SV40 poly(A) Signal ........ 2120-2170 Ampicillin Resistance Gene ........ 2798-3658 pUC Origin ........ 3807-4449

Sequencing Primer: CMV-F and IRES-R

Multiple Cloning Sites 541 ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCTCTA 601 GACTGGATCC TCGAATTCGG TACCTCAAGC TTGCGGCCGC CCCCCCCC >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> 1201 AAAACACGAT GATAATATGG CCACAACCAT GGTCGAGGCC ACCATGGTGA GCAAGGGCGA 1261 GGAGACCACA ATGGGCGTAA TCAAGCCCGA CATGAAGATC AAGCTGAAGA TGGAGGGCAA >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> 1921 CTCCACCGAC GGCATGGACG AGCTGTACAA GTAA

IRES

GFP

pmTFP1-IRES (ABP-FP-TIRES

CMV Promoter ........ 1-589 Multiple Cloning Sites ........ 591-652 IRES ........ 653-1243 mTFP1 ........ 1244-1954 SV40 poly(A) Signal ........ 2120-2170 Ampicillin Resistance Gene ........ 2798-3658 pUC Origin ........ 3807-4449

Sequencing Primer: CMV-F and IRES-R

Multiple Cloning Sites 541 ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCTCTA 601 GACTGGATCC TCGAATTCGG TACCTCAAGC TTGCGGCCGC AGATCTCTCG AGCCCCCCCC >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> 1201 AAAACACGAT GATAATATGG CCACAACCAT GGTCGAGGCC ACCATGGTGA GCAAGGGCGA 1261 GGAGACCACA ATGGGCGTAA TCAAGCCCGA CATGAAGATC AAGCTGAAGA TGGAGGGCAA >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> 1921 CTCCACCGAC GGCATGGACG AGCTGTACAA GTAA

IRES

TFP

pLanRFP-IRES (ABP-FP-RIRES

CMV Promoter ........ 1-589 Multiple Cloning Sites ........ 591-652 IRES ........ 653-1243 LanRFP ........ 1244-1957 SV40 poly(A) Signal ........ 2123-2173 Ampicillin Resistance Gene ........ 2801-3661 pUC Origin ........ 3810-4452

Sequencing Primer: CMV-F and IRES-R

Multiple Cloning Sites 541 ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCTCTA 601 GACTGGATCC TCGAATTCGG TACCTCAAGC TTGCGGCCGC AGATCTCTCG AGCCCCCCCC >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> 1201 AAAACACGAT GATAATATGG CCACAACCAT GGTCGAGGCC ACCATGGTGA GCAAGGGCGA 1261 GGAGGATAAC ATGGCCCCTC TCCCAGCAAC CCACGATTTA CACATCTCCG GCTCAATCAA >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> 1921 CCAGGACATC GCCGGCATGG ACGAGCTGTA CAAGTAA

IRES

RFP

pLanYFP-IRES (ABP-FP-YIRES

CMV Promoter ........ 1-589 Multiple Cloning Sites ........ 591-652 IRES ........ 653-1243 LanYFP ........ 1244-1954 SV40 poly(A) Signal ........ 2120-2170 Ampicillin Resistance Gene ........ 2798-3658 pUC Origin ........ 3807-4449

Sequencing Primer: CMV-F and IRES-R

Multiple Cloning Sites 541 ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCTCTA 601 GACTGGATCC TCGAATTCGG TACCTCAAGC TTGCGGCCGC AGATCTCTCG AGCCCCCCCC >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> 1201 AAAACACGAT GATAATATGG CCACAACCAT GGTCGAGGCC ACCATGGTGA GCAAGGGCGA 1261 GGAGGATAAC ATGGCCTCTC TCCCAGCGAC ACATGAGTTA CACATCTTTG GCTCCTTCAA >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> 1921 CGATGTGATG GGCATGGACG AGCTGTACAA GTAA

IRES

YFP