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Tables and Figures

Tables and Figures

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Published by: lorelie17 on Nov 01, 2010
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11/01/2010

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Table 1
Characteristics of Intestinal Amebae Visible in Different Types of Fecal Preparations
 
UNSTAINEDTEMPORARY STAINSPERMANENT STAINS
CharacteristicSaline
 
Formalin
 
Iodine (Cysts)BufferedMethylene Blue(Trophs)
1
 
Trophozoites
Motility+Cytoplasm
 
Appearance++
 
++Inclusions (rbc., bacteria)++
 
++Nucleus +
2
 
++
Cysts
Nuclei++
 
+
 
Chromatoid bodies+++
3
 
+
 
Glycogen+
 
(vacuole present)
 
1
Quensel's stain may be substituted for buffered methylene blue.
2
Nuclei of trophozoites are visible in formalin-fixed material but are usually not sufficiently distinctive for species identification.
3
Chromatoid bodies are more easily seen in unstained wet mounts than in iodine preparations
.
 
 
Table 2
Characteristics of Intestinal Flagellates, Ciliate, and Coccidia Visible in Different Types of Fecal Preparations
 
UNSTAINEDTEMPORARY STAINSPERMANENT STAINS
CharacteristicSalineFormalinIodine(Cysts)Neutral Red
1
(Trophs)
 
Flagellates
Trophozoites
 
Motility+
 
+
 
Shape+
 
+
 
+
 
+(may be distorted)Nucleus
 
+
 
+
 
+Flagella
±
 
+
 
±
Other features
2
+
 
+
 
+
 
+Cysts
 
Shape+
 
++
 
+Nuclei
 
++
 
+Fibrils
±
 
++
 
+
Ciliate
(Balantidium coli)
Trophozoites
 
Motility+
 
+
 
Macronucleus+
 
+
 
+
 
+
 
Cilia+
 
+
 
+
 
+Cysts
 
Macronucleus+
 
+
±
 
+
Coccidia
Oocysts/Sporocysts
 
+
 
++
 
±
3
 
1
Neutral red dye in methocel solutions.
2
The undulating membrane of 
Trichomonas
and the spiral groove of 
Chilomastix 
may not be visible in all cases.
3
Cryptosporidium
oocysts can be demonstrated in acid-fast stains.

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