Characteristics of Intestinal Amebae Visible in Different Types of Fecal Preparations
UNSTAINEDTEMPORARY STAINSPERMANENT STAINS
Iodine (Cysts)BufferedMethylene Blue(Trophs)
++Inclusions (rbc., bacteria)++
Quensel's stain may be substituted for buffered methylene blue.
Nuclei of trophozoites are visible in formalin-fixed material but are usually not sufficiently distinctive for species identification.
Chromatoid bodies are more easily seen in unstained wet mounts than in iodine preparations