Welcome to Scribd, the world's digital library. Read, publish, and share books and documents. See more
Download
Standard view
Full view
of .
Look up keyword
Like this
2Activity
0 of .
Results for:
No results containing your search query
P. 1
Media Biochemical Tests

Media Biochemical Tests

Ratings: (0)|Views: 294|Likes:
Published by lorelie17

More info:

Categories:Types, School Work
Published by: lorelie17 on Nov 01, 2010
Copyright:Attribution Non-commercial

Availability:

Read on Scribd mobile: iPhone, iPad and Android.
download as PPT, PDF, TXT or read online from Scribd
See more
See less

11/01/2010

pdf

text

original

 
Media & Biochemical TestsMedia & Biochemical Tests
Laboratory Objectives
 
Tests To KnowTests To Know
C
ase Study Tests
IndoleMethyl Red/Voges Proskauer 
C
itrateH
2
S production in SIMUrea hydrolysisMotilityLactose fermentationSucrose fermentationGlucose fermentation & gas production
Staphylococcus identification tests
BAPMSAMstaph broth
C
oagulase
 
I
ndole Test
I
ndole Test
How to Perform Test:
Inoculate Tryptone broth with inoculating loop.
Property it tests for:
This test is performed to help differentiate species of the family Enterobacteriaceae. It tests for the bacteria species¶ ability toproduce indole. Bacteria use an enzyme, tryptophanase to break down theamino acid, tryptophan, which makes by-products, of which, indole is one.
Media and Reagents Used:
Tryptone broth contains tryptophan.Kovac¶s reagent²contains hydrochloric acid, dimethylaminobenzaldehyde,and amyl alcohol²yellow in color.
Reading Results:
Kovac¶s reagent reacts with indole and creates a redcolor at the top part of the test tube.

Activity (2)

You've already reviewed this. Edit your review.
1 thousand reads
1 hundred reads

You're Reading a Free Preview

Download
scribd
/*********** DO NOT ALTER ANYTHING BELOW THIS LINE ! ************/ var s_code=s.t();if(s_code)document.write(s_code)//-->