pORB-MCS1-sIRES-MCS2

Cat#: ABP-BM-sIRESMC
CAG Promoter
* For efficient mammalian expression, including CMV
663-1702
T he pORB-MCS1-sIRES-MCS2 vector contains an
insect internal ribosome entry site (IRES) from the
white spot syndrome virus (WSSV) of marine shrimp,
ie enhancer,Chicken β actin promoter, Exon1, Intron and
which is much stronger than what is used in Allele’s
partial Exon2. current pAcIRES vector, the IRES from cricket paralysis
virus (CrPV). By including a selection marker / reporter
gene in the expression cassette you can save time and
Polh Promoter 1729-1823 eliminate clonal selection because virtually all transfected
* For efficient insect cell expression
cells expressing the selection marker also express your
gene of interest. This IRES-mediated translation can start
at methionine, providing more freedom for cDNA insertion
sIRES 1920-2099 than pAcIRES.
* From WSSV for strong insect cell expression

Poly(A) signal 2259-2465

* Rabbit β globin terminator

CoIE origin 5295-5934

AmpR 6081-6941

F or Research Use Only. Not for Diagnostic or

Therapeutic Use. Purchase does not include or
carry any right to resell or transfer this product either
as a stand-alone product or as a component of another
product. Any use of this product other than the permitted
use without the express written authorization of Allele
Biotech is strictly prohibited

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