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  • H.W. Buffer Preparation Problems

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    Michelle Coleman
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    Show, in a logical order, the calculations to make 1 liter of 0.01M phosphate buffer pH 5.8. Be sure to include all units. PH a beaker is a cylindrical thick walled container used for mixing chemicals with a magnetic stir bar and for pouring chemicals. When and why do you use An Erlenmeyer flask? volumetric flasks are used to make solutions of known a concentration by the dilution of a more concentrated solution.

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    Show, in a logical order, the calculations to make 1 liter of 0.01M phosphate buffer pH 5.8. Be sure to include all units. PH a beaker is a cylindrical thick walled container used for mixing chemicals with a magnetic stir bar and for pouring chemicals. When and why do you use An Erlenmeyer flask? volumetric flasks are used to make solutions of known a concentration by the dilution of a more concentrated solution.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as DOCX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    264 views3 pages

    H.W. Buffer Preparation Problems

    Uploaded by

    Michelle Coleman
    Show, in a logical order, the calculations to make 1 liter of 0.01M phosphate buffer pH 5.8. Be sure to include all units. PH a beaker is a cylindrical thick walled container used for mixing chemicals with a magnetic stir bar and for pouring chemicals. When and why do you use An Erlenmeyer flask? volumetric flasks are used to make solutions of known a concentration by the dilution of a more concentrated solution.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as DOCX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 3

    Æ 

    
    
    
    Work out the problem below for homework. Do your work on a separate page before writing your answers here.
    Show, in a logical order, the calculations to make 1 liter of 0.01M phosphate buffer pH 5.8. Be sure to include all units.

      ‘
    
     ½  ‘‘‘
    ‘ ‘  ‘‘ ‘ ‘ ‘‘‘‘  ‘‘‘
    ‘ 
    
     ‘‘‘‘   ‘‘ ‘‘‘‘‘‘‘‘‘‘‘‘‘ ‘  ‘‘ ‘‘ ‘
    ‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘
    ‘‘‘‘‘‘‘‘‘‘‘‘‘ ‘‘‘‘‘
    ‘ ‘‘
    ‘   ‘‘ ‘ ‘ ‘
    ‘
    ‘‘‘‘‘‘‘‘‘‘½  ‘‘ ‘‘  ‘ ‘
    ‘ ‘ ‘‘ ‘‘
      
     
    Æ  
       
     ^ .0 8.0 ‘ 5.8
        ‘%‘&   ‘

    ‘ ‘‘!"‘#$ ‘%‘&‘ ‘‘"‘# ‘

    When and why do you use a beaker?


    '‘
     ‘ ‘ ‘&   ‘ 
    ‘ ‘  ‘‘ ‘  (‘  ‘ ‘ ‘ ( ‘
     ‘ ‘ ‘ ‘ (‘  ‘

    When and why do you use an Erlenmeyer flask?


    '‘)  & ‘ 
    ‘ ‘ &‘‘ ‘ ‘   (*‘ (‘ ‘  (‘‘ + ‘,‘
     ‘  ‘
    ‘  ‘ ‘ ‘ ‘ 
    ‘‘  (‘ ‘‘-   *‘‘ ‘ ‘ ‘
     ‘‘ 
    ‘‘ ‘‘

    When and why do you use a volumetric flask?


      ‘ 
    ‘ ‘‘‘
    ‘ ‘‘
    ‘ ‘  ‘&‘‘  ‘‘ ‘
     ‘ ‘ ‘,&‘ ‘   ‘‘& ‘- &‘  ‘ ‘‘ + ‘

    Describe briefly, in their proper sequence, the main steps required to make up 0.5 liters of 0.01M phosphate buffer pH
    6.6 (include your actual calculations and the glassware required etc.)?
    ‘
    ‘½  ‘‘‘
    ‘ ‘‘ ‘ ‘ ‘  ‘‘‘
    ‘ 
    
     ‘‘‘‘ .‘‘ ‘‘‘‘‘‘‘‘‘‘‘‘‘ ‘ ‘‘ ‘ ‘
    ‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘
    ‘‘‘‘‘‘‘‘‘‘‘‘‘ ‘‘‘‘‘
    ‘ ‘‘
    ‘ ‘‘ ‘ ‘ ‘
    ‘
    ‘‘‘‘‘‘‘‘‘‘½  ‘‘ ‘‘  ‘ ‘
    ‘ ‘ ‘‘$$ ‘‘
       1 ‘2 ‘, ‘
     ‘ &‘ ‘
     6 .5 · .5 6.6
        ‘%‘&   ‘
    
    ‘$"‘ ‘‘ $‘ ‘%‘&‘/0"‘‘ ‘‘!# ‘‘

    c‘
    ‘‘‘‘‘‘‘
    ‘
    / 3 (‘ ‘ ‘(  ‘&  ‘   ‘‘‘ ‘‘ ‘
    ‘ ‘ ‘ ‘  ‘
    
     ‘ ‘ (‘ ‘'‘/‘ ‘‘  ‘  ‘456‘‘ ‘½
    ‘‘ ‘‘  ‘
    ‘ 7‘‘ (‘‘$$‘
    # 3‘ ‘‘‘ ‘‘ ‘ ‘‘
     ‘‘ ‘‘ ‘-  ‘ 
    ‘ ‘ (‘‘- ‘
     (‘5‘ ‘   ‘‘ ‘‘ ‘ (‘ ‘ ‘1  ‘ ‘
     5‘ ‘‘ ‘- ‘ ‘ ‘ ‘ ‘4   *‘ *‘‘ ‘6‘‘ ‘( ‘‘
     ‘ ‘

    ë‘
    {
    

     
    
    
    
    1.‘ How much dibasic sodium phosphate 1 H  would one have to weigh out to produce 1 liter of a 1M
    solution?____________.
    ‘
    89/(‘‘
    ‘ ‘.‘‘ ‘ ‘ ‘
    ‘
    . How much dibasic sodium phosphate H  would one have to weigh out to produce 1 liter of a 1M solution?‘
    ‘‘‘‘‘‘‘‘‘‘‘
    ‘‘‘‘‘‘‘‘89$(‘‘
    ‘ .‘‘ ‘ ‘‘
    ‘
    ·. Do both of the solutions above have the same number of phosphate molecules?‘
    ‘
      ‘  ‘ ‘: 15#‘;‘   ‘  ‘ ‘: 15#‘‘ -‘‘‘ ‘
      ‘‘ ‘ ‘ ‘ ‘‘  ‘‘: ‘ ‘‘ ‘ ‘   ‘‘‘
     ‘
    ‘
    4. What is the final concentration that results when you dilute 100 mL of 0. M sodium phosphate to a final volume of 1000
    mL ?_________.

    ‘‘‘‘‘‘‘‘‘‘‘‘ ‘‘‘
    ‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘ ‘
    ‘
    ‘5. How would you make up 50 mL of a 0.05M sodium phosphate buffer pH 6.8?‘  ‘
    
    ‘½  ‘‘‘
    ‘ ‘‘‘ ‘ ‘  ‘‘‘
    ‘ 
    
     ‘‘‘‘ .‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘ ‘ ‘‘‘$ ‘
    ‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘‘
    ‘‘‘‘‘‘‘‘‘‘‘‘‘$ ‘‘‘‘‘
    ‘ ‘‘
    ‘ ‘‘‘ ‘ ‘
    ‘
    ‘‘‘‘‘‘‘‘‘‘½  ‘‘ ‘‘  ‘ ‘
    ‘ ‘ ‘‘$ ‘‘
       1 ‘2 ‘, ‘
     ‘ &‘ ‘
     51.0 4^.0 6.8
        ‘%‘&   ‘
    
    ‘ "‘‘$ ‘‘/ ! %‘&‘#!"‘‘$ ‘‘/$‘
    ‘‘‘‘‘‘‘
    /‘‘3 (‘ ‘  ‘(  ‘&  ‘   ‘‘‘ ‘‘ ‘
    ‘ ‘ ‘ ‘# ‘‘‘‘‘‘
    
     ‘ ‘ (‘ ‘'‘  ‘‘  ‘  ‘456‘‘ ‘½
    ‘‘ ‘‘  ‘‘‘‘
    ‘ 7‘‘ (‘‘$‘
    # 3‘ ‘‘‘ ‘‘ ‘ ‘‘
     ‘‘ ‘‘ ‘-  ‘ 
    ‘ ‘ (‘‘- ‘
     (‘5‘
     5‘ ‘‘ ‘- ‘ ‘ ‘ ‘ ‘4   *‘ *‘‘ ‘6‘‘ ‘‘ ‘
     ‘

    ·‘

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