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T-DNA Mediated Gene Transfer in Plants

T-DNA Mediated Gene Transfer in Plants

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Published by Sathiyaraj
explains about the tDNA mediated gene transfer in plant using agrobacteriam .. Science and technology
explains about the tDNA mediated gene transfer in plant using agrobacteriam .. Science and technology

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Published by: Sathiyaraj on Oct 26, 2007
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05/08/2014

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T-DNA mediated Gene Transfer in Plants 
(
T-DNA
 
식물에서
 
)
Sathiyaraj Srinivasan
 
사티야라스리니바
 Dept. of Oriental Medicinalmaterials and ProcessingKyung Hee University,Republic of Korea – 449 701
 
 
Gene Transfer Technique
To transfer a gene from one DNA molecule to another DNA molecule.
Gene Transfer includes isolation of gene, manipulation (target geneinsertion ) and reintroduction of DNA into the cells or model organisms.
It used to make a crop to resistant to a particular herbicide, pest, weedsor introducing a novel trait, or producing a new protein or enzyme.
The gene transfer methods normally include three categories:1. transduction by biochemical methods,2. transduction by physical methods,3. virus-mediated transduction.
The gene transfer results can be transient and stable transduction.
 
 
Transfection Types:
Transient transfection 
In transient transfection, the transfected DNA is not integrated intohost chromosome. DNA is transferred into a recipient cell in order to obtain atemporary but high level of expression of the target gene.Fast, flexible, not influenced by position effects, can provide largeamounts of protein for detailed characterization, does not require regenerationsystem, gene expression limited to specific plant tissues.
Stable transfection
Stable transfection is also called permanent transfection. By the stabletransfection, the transferred DNA is integrated (inserted) into chromosomalDNA and the genetics of recipient cells is permanent changed.Time consuming, labor intensive, influenced by position effects, largeproduction volumes, requires regeneration system, large amounts of glasshouse space, gene expression in all plant tissues

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Ashutosh Singh added this note
in this article i understand the basics of t dna transformation thanks the authur nd publisher of site
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