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enzyme biotechnology by Dr S M bhatt Amity, http://processtechnology.wetpaint.com

enzyme biotechnology by Dr S M bhatt Amity, http://processtechnology.wetpaint.com

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Published by smbhatt
for more information please also
see my web page http://processtechnology.wetpaint.com
with
thanks
Dr. S. M. Bhatt
Amity University
Noida
Mob-9313993840
for more information please also
see my web page http://processtechnology.wetpaint.com
with
thanks
Dr. S. M. Bhatt
Amity University
Noida
Mob-9313993840

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Categories:Types, Research
Published by: smbhatt on Jul 30, 2008
Copyright:Attribution Non-commercial

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05/09/2014

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Dr. S. M. Bhatt smbhatt@amity.edu , smbhatt_bhu@rediffmail.com phone 9313993840Amity University Uttar PradeshENZYMOLOGY AND ENZYME TECHNOLOGYCourse Code:BTBBT 30602Course Objective:The course aims to provide an understanding of the principles and application ofproteins, secondary metabolites and enzyme biochemistry in therapeuticapplications and clinical diagnosis. The theoretical understanding of biochemicalsystems would certainly help to interpret the results of laboratory experiments.Course Contents:Module IEnzymes: Introduction and scope, Nomenclature, Mechanism of Catalysis.Module IISpecificity of enzyme action, monomeric and oligomeric enzymes, Enzyme inhibition.Module IIIEnzyme Kinetics: Single substrate steady state kinetics; Michaelis Mentenequation, Linear plots, King-Altman’s method; Inhibitors and activators;Multisubstrate systems; ping-pong mechanism, Alberty equation, Sigmoidal kineticsand Allosteric enzymesModule IVExtraction & purification of enzymes.Module VImmobilization of Enzymes; Advantages, Carriers, adsorption, covalent coupling,cross-linking and entrapment methods, Micro-environmental effects.Module VIBiotechnological applications of enzymes: Large scale production and purificationof enzymes, enzyme utilization in industry, enzymes and recombinant DNA technologyExamination Scheme:Component CodesH/QSCT2EEWeight age (%) 10102060Text & References:TextBiotechnological Innovations in Chemical Synthesis, R.C.B. Currell, V.D.Mieras, Biotol Partners Staff, Butterworth Heinemann.Enzyme Technology, M.F. Chaplin and C. Bucke, Cambridge University Press.Enzymes: A Practical Introduction to Structure, Mechanism and Data Analysis,R.A. Copeland, John Wiley and Sons Inc.ReferencesEnzymes Biochemistry, Biotechnology, Clinical Chemistry, Trevor PalnerDr. S. M. Bhatt smbhatt@amity.edu , smbhatt_bhu@rediffmail.com phone 9313993840Amity University Uttar PradeshEnzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady StateEnzyme Systems, I.H. Segel, Wiley-Interscience
 
Industrial Enzymes & their applications, H. Uhlig, John Wiley and Sons Inc. ENZYMOLOGY AND ENZYME TECHNOLOGY LABCourse Code:BTBPL 30622Course Objective:The laboratory will help the students to isolate enzymes from different sources,enzyme assays and studying their kinetic parameters which have immense importancein industrial processes.Course Contents:Module IIsolation of enzymes from plant and microbial sources.Module IIEnzyme assay; activity and specific activity – determination of amylase, nitratereductase, cellulase, proteaseModule IIIPurification of Enzyme by ammonium sulphate fractionationModule IVEnzyme Kinetics: Effect of varying substrate concentration on enzyme activity,determination of Michaelis-Menten constant (Km) and Maximum Velocity (Vmax.) usingLineweaver-Burk plot.Module VEffect of Temperature and pH on enzyme activityModule VIEnzyme immobilizationExamination Scheme:Major Experiments:40Minor Experiments:20Spotting:10Viva:20Records:10Total:100 Note: Minor variation could be there depending on the examiner.Text:Practical Biochemistry, Sawhney and SinghChapter- 1Enzymes an introduction9DefinitionIntroduction
 
Historical aspects in enzyme discoveryClassification of Enzymes.application of enzymesChapter-2 Types of Enzymes16A. Simple enzymesB. Complex enzymes Complex enzymesRole of CoenzymesA. CatalystsB. Biocatalyst or EnzymesHow enzymes speed up the reaction without taking partUnit of activityChapter 3Enzyme Specificity20ENZYME MECHANISM MODELSA. Lock and Key Model for Enzyme ActivityB. Induced Fit (Hand and Glove) Model of Enzyme ActivityMechanisms of Enzyme CatalysisChapter 4 Enzyme-Substrate Interactions 24a. Catalysis by Bond Strainb. Catalysis by Proximity and Orientationc. Catalysis Involving Proton Donors (Acids) and Acceptors (Bases)Concerted acid-base catalysisd. Covalent Catalysis:Covalent Intermediatese) Metal Ion CatalysisCatalytic Powera) Proximityb) Orientationf. Catalysis in organic solventsSome advantages to using enzymes in non-aqueous solvents include (Dordick, 1989):Importance and relevance of whole cell enzymes in biotechnologyThere may be some drawbacks to using whole cells in catalysis. These includeAbzymesExercisesChapter-5 ENZYME KINETICS & INHIBITION46Chemical Reactions and RatesChemical Reaction OrderMichaelis-Menton constanta. Pre-steady state:--b. Steady state:--Derivation:Catalytic efficiency and Turnover number.Chapter-6Factor affecting the rate of catalysis 58Substrate concentrationEffect of temperature and pressureEffect of pH on EnzymeChapter-7Enzyme inhibition68A. Competitive inhibitionB. Uncompetitive inhibitionC. Noncompetitive inhibitionDetermination of Vmax and KmChapter-8Multisubstrate enzymes83Enzyme Catalysis

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