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Russell
CHAPTER 14
Gene Mapping In Bacteria and
Bacteriophages
d. A double crossover event occurs, replacing one recipient DNA strand with the donor strand.
e. The recipient now has a region of heteroduplex DNA. One strand has the recipient’s original a allele and the
other strand has the new a+ allele.
f. DNA replication will produce one chromosome with the original (a) genotype, and one with the recombinant
(a+) genotype.
g. The cell with the recombinant genotype is then selected by its phenotypic change.
b. Most pairs of rII mutants produced r+ recombinants when crossed, and so had changes in different base pairs
within the gene (heteroallelic).
c. Lowest detectible recombination frequency was 0.01%. Correlating this with the T4 genetic map, it was
calculated that the smallest recombination distance observed was about three base pairs.
3. Later experiments have observed recombination between adjacent base pairs, indicating that the base
pair is both the unit of mutation and the unit of recombination. This replaced the older idea that the
gene was indivisible.