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Cytochemical Stain

Cytochemical Stain

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Published by dreyngerous

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Published by: dreyngerous on Aug 03, 2008
Copyright:Attribution Non-commercial

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11/12/2013

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2006200368200620036620068386652006838514200683839320068383792006838507
 
LEUKOCYTE ALKALINEPHOSPHATASE (LAP)
LAP is useful for differentiating CML from leukemoidreaction.
PRINCIPLE
LAP is enzyme found in secondary granules of neutrophils.The substrate naphthol AS-BI phosphate is hydrolyzed bythe enzyme at an alkaline pH.This hydrolyzed substrate in combination with a dye such asfast red violet LB or fast blue BB produces a coloredprecipitate at site of the enzyme activity.
 
SCORING
LAP activity is scored in the maturepolymorphonuclear cells and bands only.The activity scores range from 0 to 4+.The scores of 100 mature polymorphonuclear cellsand bands are added for the LAP score.

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